1e0r: Difference between revisions

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New page: left|200px<br /><applet load="1e0r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e0r, resolution 2.8Å" /> '''BETA-APICAL DOMAIN OF...
 
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[[Image:1e0r.jpg|left|200px]]<br /><applet load="1e0r" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1e0r, resolution 2.8&Aring;" />
'''BETA-APICAL DOMAIN OF THERMOSOME'''<br />


==Overview==
==Beta-apical domain of thermosome==
The crystal structure of the beta-apical domain of the thermosome, an, archaeal group II chaperonin from Thermoplasma acidophilum, has been, determined at 2.8 A resolution. The structure shows an invariant globular, core from which a 25 A long protrusion emanates, composed of an elongated, alpha-helix (H10) and a long extended stretch consisting of residues, GluB245-ThrB253. A comparison with previous apical domain structures, reveals a large segmental displacement of the protruding part of helix H10, via the hinge GluB276-ValB278. The region comprising residues, GluB245-ThrB253 adopts an extended beta-like conformation rather than the, alpha-helix seen in the alpha-apical domain. Consequently, it appears that, the protrusions of the apical domains from group II chaperonins might, assume a variety of context-dependent conformations during an open, substrate-accepting state of the chaperonin. Sequence variations in the, protrusion regions that are found in the eukaryotic TRiC/CCT subunits may, provide different structural propensities and hence serve different roles, in substrate recognition.
<StructureSection load='1e0r' size='340' side='right'caption='[[1e0r]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1e0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E0R FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e0r OCA], [https://pdbe.org/1e0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e0r RCSB], [https://www.ebi.ac.uk/pdbsum/1e0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e0r ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/THSB_THEAC THSB_THEAC] Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/1e0r_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e0r ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the beta-apical domain of the thermosome, an archaeal group II chaperonin from Thermoplasma acidophilum, has been determined at 2.8 A resolution. The structure shows an invariant globular core from which a 25 A long protrusion emanates, composed of an elongated alpha-helix (H10) and a long extended stretch consisting of residues GluB245-ThrB253. A comparison with previous apical domain structures reveals a large segmental displacement of the protruding part of helix H10 via the hinge GluB276-ValB278. The region comprising residues GluB245-ThrB253 adopts an extended beta-like conformation rather than the alpha-helix seen in the alpha-apical domain. Consequently, it appears that the protrusions of the apical domains from group II chaperonins might assume a variety of context-dependent conformations during an open, substrate-accepting state of the chaperonin. Sequence variations in the protrusion regions that are found in the eukaryotic TRiC/CCT subunits may provide different structural propensities and hence serve different roles in substrate recognition.


==About this Structure==
Crystal structure of the beta-apical domain of the thermosome reveals structural plasticity in the protrusion region.,Bosch G, Baumeister W, Essen LO J Mol Biol. 2000 Aug 4;301(1):19-25. PMID:10926489<ref>PMID:10926489</ref>
1E0R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E0R OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of the beta-apical domain of the thermosome reveals structural plasticity in the protrusion region., Bosch G, Baumeister W, Essen LO, J Mol Biol. 2000 Aug 4;301(1):19-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10926489 10926489]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1e0r" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Chaperonin 3D structures|Chaperonin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
[[Category: Baumeister, W.]]
[[Category: Baumeister W]]
[[Category: Bosch, G.]]
[[Category: Bosch G]]
[[Category: Essen, L.O.]]
[[Category: Essen L-O]]
[[Category: chaperonin]]
[[Category: groel]]
[[Category: hsp60]]
[[Category: tcp1]]
[[Category: thermoplasma acidophilum]]
[[Category: thermosome]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:43:05 2007''

Latest revision as of 08:09, 6 December 2023

Beta-apical domain of thermosome

1e0r, resolution 2.80Å

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