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New page: left|200px<br /><applet load="1ebf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ebf, resolution 2.30Å" /> '''HOMOSERINE DEHYDROGE...
 
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[[Image:1ebf.jpg|left|200px]]<br /><applet load="1ebf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ebf, resolution 2.30&Aring;" />
'''HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+'''<br />


==Overview==
==HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+==
The structure of the antifungal drug target homoserine dehydrogenase (HSD), was determined from Saccharomyces cerevisiae in apo and holo forms, and as, a ternary complex with bound products, by X-ray diffraction. The three, forms show that the enzyme is a dimer, with each monomer composed of three, regions, the nucleotide-binding region, the dimerization region and the, catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the, Rossmann fold. The novel folds impose a novel composition and arrangement, of active site residues when compared to all other currently known, oxidoreductases. This observation, in conjunction with site-directed, mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
<StructureSection load='1ebf' size='340' side='right'caption='[[1ebf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ebf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EBF FirstGlance]. <br>
1EBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NA and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EBF OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ebf OCA], [https://pdbe.org/1ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ebf RCSB], [https://www.ebi.ac.uk/pdbsum/1ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ebf ProSAT]</span></td></tr>
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10700284 10700284]
</table>
[[Category: Homoserine dehydrogenase]]
== Function ==
[https://www.uniprot.org/uniprot/DHOM_YEAST DHOM_YEAST]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/1ebf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ebf ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Berghuis AM]]
[[Category: Berghuis, A.M.]]
[[Category: DeLaBarre B]]
[[Category: DeLaBarre, B.]]
[[Category: Thompson PR]]
[[Category: Thompson, P.R.]]
[[Category: Wright GD]]
[[Category: Wright, G.D.]]
[[Category: NA]]
[[Category: NAD]]
[[Category: dehydrogenase]]
[[Category: dimer]]
[[Category: dinucleotide]]
[[Category: homoserine]]
[[Category: nad]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:52:31 2007''

Latest revision as of 07:00, 7 February 2024

HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+

1ebf, resolution 2.30Å

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