1es9: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1es9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1es9, resolution 1.3Å" /> '''X-RAY CRYSTAL STRUCTU...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(17 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1es9.jpg|left|200px]]<br /><applet load="1es9" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1es9, resolution 1.3&Aring;" />
'''X-RAY CRYSTAL STRUCTURE OF R22K MUTANT OF THE MAMMALIAN BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASES (PAF-AH)'''<br />


==Overview==
==X-RAY CRYSTAL STRUCTURE OF R22K MUTANT OF THE MAMMALIAN BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASES (PAF-AH)==
The mammalian brain contains significant amounts of the cytosolic isoform, Ib of the platelet-activating factor acetylhydrolase (PAF-AH), a unique, type of PLA2. This oligomeric protein complex contains three types of, subunits: two homologous (63% identity) 26 kDa catalytic subunits, (alpha(1) and alpha(2)) which harbor all the PAF-AH activity, and the 45, kDa beta-subunit (LIS1), a product of the causal gene for Miller-Dieker, lissencephaly. During fetal development, the preferentially expressed, alpha(1)-subunit forms a homodimer, which binds to a homodimer of LIS1, whereas in adult organisms alpha(1)/alpha(2) and alpha(2)/alpha(2) dimers, also bound to dimeric LIS1, are the prevailing species. The consequences, of this "switching" are not understood, but appear to be of physiological, significance. The alpha(1)- and alpha(2)-subunits readily associate with, very high affinity to form homodimers. The nature of the interface has, been elucidated by the 1.7 A resolution crystal structure of the, alpha(1)/alpha(1) homodimer (Ho et al., 1997). Here, we examined the, functional consequences of the dimerization in both types of, alpha-subunits. We obtained monomeric protein in the presence of high, concentrations (&gt;50 mM) of Ca2+ ions, and we show that it is catalytically, inactive and less stable than the wild type. We further show that Arg29, and Arg22 in one monomer contribute to the catalytic competence of the, active site across the dimer interface, and complement the catalytic triad, of Ser47, Asp192 and His195, in the second monomer. These results indicate, that the brain PAF-acetylhydrolase is a unique PLA2 in which dimerization, is essential for both stability and catalytic activity.
<StructureSection load='1es9' size='340' side='right'caption='[[1es9]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1es9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ES9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ES9 FirstGlance]. <br>
1ES9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/1-alkyl-2-acetylglycerophosphocholine_esterase 1-alkyl-2-acetylglycerophosphocholine esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.47 3.1.1.47] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ES9 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1es9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1es9 OCA], [https://pdbe.org/1es9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1es9 RCSB], [https://www.ebi.ac.uk/pdbsum/1es9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1es9 ProSAT]</span></td></tr>
==Reference==
</table>
The functional implications of the dimerization of the catalytic subunits of the mammalian brain platelet-activating factor acetylhydrolase (Ib)., McMullen TW, Li J, Sheffield PJ, Aoki J, Martin TW, Arai H, Inoue K, Derewenda ZS, Protein Eng. 2000 Dec;13(12):865-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11239086 11239086]
== Function ==
[[Category: 1-alkyl-2-acetylglycerophosphocholine esterase]]
[https://www.uniprot.org/uniprot/PA1B3_BOVIN PA1B3_BOVIN] Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/1es9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1es9 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Aoki, J.]]
[[Category: Aoki J]]
[[Category: Arai, H.]]
[[Category: Arai H]]
[[Category: Derewenda, Z.S.]]
[[Category: Derewenda ZS]]
[[Category: Inoue, K.]]
[[Category: Inoue K]]
[[Category: Li, J.]]
[[Category: Li J]]
[[Category: Martin, T.W.]]
[[Category: Martin TW]]
[[Category: McMullen, T.W.P.]]
[[Category: McMullen TWP]]
[[Category: Sheffield, P.J.]]
[[Category: Sheffield PJ]]
[[Category: alpha/beta hydrolase fold]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:16:15 2007''
Retrieved from "https://proteopedia.org/1es9"