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New page: left|200px<br /><applet load="1ev7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ev7, resolution 2.38Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1ev7.gif|left|200px]]<br /><applet load="1ev7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ev7, resolution 2.38&Aring;" />
'''CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI'''<br />


==Overview==
==CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI==
NAE:I is transformed from DNA endonuclease to DNA topoisomerase and, recombinase by a single amino acid substitution. The crystal structure of, NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric, molecule with two domains per monomer. Each domain contains one potential, DNA recognition motif corresponding to either endonuclease or, topoisomerase activity. The N-terminal domain core folds like the other, type II restriction endonucleases as well as lambda-exonuclease and the, DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and, catalytic mechanism. The C-terminal domain contains a catabolite activator, protein (CAP) motif present in many DNA-binding proteins, including the, type IA and type II topoisomerases. Thus, the NAE:I structure implies that, DNA processing enzymes evolved from a few common ancestors. NAE:I may be, an evolutionary bridge between endonuclease and DNA processing enzymes.
<StructureSection load='1ev7' size='340' side='right'caption='[[1ev7]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref>


==About this Structure==
==See Also==
1EV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase., Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H, EMBO J. 2000 Jun 15;19(12):3110-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10856254 10856254]
__TOC__
[[Category: Lechevalieria aerocolonigenes]]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Lentzea aerocolonigenes]]
[[Category: Chen, Y.]]
[[Category: Chen Y]]
[[Category: Colandene, J.D.]]
[[Category: Colandene JD]]
[[Category: Huai, Q.]]
[[Category: Huai Q]]
[[Category: Luo, F.]]
[[Category: Luo F]]
[[Category: Zhao, Y.]]
[[Category: Zhao Y]]
[[Category: apo-naei]]
[[Category: cap]]
[[Category: helix-turn-helix]]
[[Category: restriction endonuclease]]
[[Category: topoisomerase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:20:42 2007''

Latest revision as of 07:07, 7 February 2024

CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI

1ev7, resolution 2.38Å

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