1ewy: Difference between revisions

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New page: left|200px<br /><applet load="1ewy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ewy, resolution 2.38Å" /> '''ANABAENA PCC7119 FER...
 
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[[Image:1ewy.jpg|left|200px]]<br /><applet load="1ewy" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ewy, resolution 2.38&Aring;" />
'''ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX'''<br />


==Overview==
==ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX==
Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor, ferredoxin (Fd) from the cyanobacterium Anabaena PCC7119 have been, co-crystallized. The unit-cell parameters are a = b = 63.72, c = 158.02 A, and the space group is P2(1)2(1)2(1). The crystal structure has been, solved with 2.4 A resolution synchrotron data by molecular replacement, anomalous dispersion and R(min) search methods. For the computations, the, crystal was treated as a merohedral twin. The asymmetric unit contains two, FNR molecules and one ferredoxin molecule. The packing of the FNR, molecules displays a nearly tetragonal symmetry (space group P4(3)2(1)2), whereas the ferredoxin arrangement is orthorhombic. This study provides, the first crystallographic model of a dissociable complex between FNR and, Fd.
<StructureSection load='1ewy' size='340' side='right'caption='[[1ewy]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ewy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7119 Nostoc sp. PCC 7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ewy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ewy OCA], [https://pdbe.org/1ewy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ewy RCSB], [https://www.ebi.ac.uk/pdbsum/1ewy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ewy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FENR_NOSSO FENR_NOSSO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ewy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ewy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor ferredoxin (Fd) from the cyanobacterium Anabaena PCC7119 have been co-crystallized. The unit-cell parameters are a = b = 63.72, c = 158.02 A and the space group is P2(1)2(1)2(1). The crystal structure has been solved with 2.4 A resolution synchrotron data by molecular replacement, anomalous dispersion and R(min) search methods. For the computations, the crystal was treated as a merohedral twin. The asymmetric unit contains two FNR molecules and one ferredoxin molecule. The packing of the FNR molecules displays a nearly tetragonal symmetry (space group P4(3)2(1)2), whereas the ferredoxin arrangement is orthorhombic. This study provides the first crystallographic model of a dissociable complex between FNR and Fd.


==About this Structure==
Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule.,Morales R, Kachalova G, Vellieux F, Charon MH, Frey M Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1408-12. PMID:11053838<ref>PMID:11053838</ref>
1EWY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nostoc_sp._pcc_7119 Nostoc sp. pcc 7119] with FAD and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EWY OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule., Morales R, Kachalova G, Vellieux F, Charon MH, Frey M, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1408-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11053838 11053838]
</div>
[[Category: Ferredoxin--NADP(+) reductase]]
<div class="pdbe-citations 1ewy" style="background-color:#fffaf0;"></div>
[[Category: Nostoc sp. pcc 7119]]
[[Category: Protein complex]]
[[Category: Charon, M.H.]]
[[Category: Frey, M.]]
[[Category: Morales, R.]]
[[Category: FAD]]
[[Category: FES]]
[[Category: electron transfer complex]]
[[Category: ferredoxin]]
[[Category: photosynthesis]]
[[Category: protein-protein interaction]]
[[Category: reductase]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:23:14 2007''
==See Also==
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nostoc sp. PCC 7119]]
[[Category: Charon MH]]
[[Category: Frey M]]
[[Category: Morales R]]

Latest revision as of 05:52, 13 August 2026

ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX

1ewy, resolution 2.38Å

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