1dc8: Difference between revisions

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{{Seed}}
[[Image:1dc8.png|left|200px]]


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==STRUCTURE OF A TRANSIENTLY PHOSPHORYLATED "SWITCH" IN BACTERIAL SIGNAL TRANSDUCTION==
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<StructureSection load='1dc8' size='340' side='right'caption='[[1dc8]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1dc8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DC8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dc8 OCA], [https://pdbe.org/1dc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dc8 RCSB], [https://www.ebi.ac.uk/pdbsum/1dc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dc8 ProSAT]</span></td></tr>
{{STRUCTURE_1dc8|  PDB=1dc8  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/NTRC_SALTY NTRC_SALTY] Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dc/1dc8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dc8 ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Receiver domains are the dominant molecular switches in bacterial signalling. Although several structures of non-phosphorylated receiver domains have been reported, a detailed structural understanding of the activation arising from phosphorylation has been impeded by the very short half-lives of the aspartylphosphate linkages. Here we present the first structure of a receiver domain in its active state, the phosphorylated receiver domain of the bacterial enhancer-binding protein NtrC (nitrogen regulatory protein C). Nuclear magnetic resonance spectra were taken during steady-state autophosphorylation/dephosphorylation, and three-dimensional spectra from multiple samples were combined. Phosphorylation induces a large conformational change involving a displacement of beta-strands 4 and 5 and alpha-helices 3 and 4 away from the active site, a register shift and an axial rotation in helix 4. This creates an exposed hydrophobic surface that is likely to transmit the signal to the transcriptional activation domain.


===STRUCTURE OF A TRANSIENTLY PHOSPHORYLATED "SWITCH" IN BACTERIAL SIGNAL TRANSDUCTION===
Structure of a transiently phosphorylated switch in bacterial signal transduction.,Kern D, Volkman BF, Luginbuhl P, Nohaile MJ, Kustu S, Wemmer DE Nature. 1999 Dec 23-30;402(6764):894-8. PMID:10622255<ref>PMID:10622255</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 10622255 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_10622255}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1DC8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DC8 OCA].
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
 
[[Category: Kern D]]
==Reference==
[[Category: Kustu S]]
Structure of a transiently phosphorylated switch in bacterial signal transduction., Kern D, Volkman BF, Luginbuhl P, Nohaile MJ, Kustu S, Wemmer DE, Nature. 1999 Dec 23-30;402(6764):894-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10622255 10622255]
[[Category: Luginbuhl P]]
[[Category: Salmonella typhimurium]]
[[Category: Nohaile MJ]]
[[Category: Single protein]]
[[Category: Volkman BF]]
[[Category: Kern, D.]]
[[Category: Wemmer DE]]
[[Category: Kustu, S.]]
[[Category: Luginbuhl, P.]]
[[Category: Nohaile, M J.]]
[[Category: Volkman, B F.]]
[[Category: Wemmer, D E.]]
[[Category: Conformational rearrangement]]
[[Category: Phosphorylation]]
[[Category: Receiver domain]]
[[Category: Signal transduction]]
[[Category: Two-component system]]
 
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Retrieved from "https://proteopedia.org/1dc8"