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New page: left|200px<br /><applet load="1ezj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ezj, resolution 1.90Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1ezj.gif|left|200px]]<br /><applet load="1ezj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ezj, resolution 1.90&Aring;" />
'''CRYSTAL STRUCTURE OF THE MULTIMERIZATION DOMAIN OF THE PHOSPHOPROTEIN FROM SENDAI VIRUS'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE MULTIMERIZATION DOMAIN OF THE PHOSPHOPROTEIN FROM SENDAI VIRUS==
The high resolution X-ray structure of the Sendai virus oligomerization, domain reveals a homotetrameric coiled coil structure with many details, that are different from classic coiled coils with canonical hydrophobic, heptad repeats. Alternatives to the classic knobs-into-holes packing lead, to differences in supercoil pitch and diameter that allow water molecules, inside the core. This open and more hydrophilic structure does not seem to, be destabilized by mutations that would be expected to disrupt classic, coiled coils.
<StructureSection load='1ezj' size='340' side='right'caption='[[1ezj]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ezj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sendai_virus_(strain_Harris) Sendai virus (strain Harris)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ezj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezj OCA], [https://pdbe.org/1ezj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ezj RCSB], [https://www.ebi.ac.uk/pdbsum/1ezj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHOSP_SENDH PHOSP_SENDH] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template.  Acts as a chaperone for newly synthesized free N protein, so-called N(0). Stabilizes the L protein upon binding it.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The high resolution X-ray structure of the Sendai virus oligomerization domain reveals a homotetrameric coiled coil structure with many details that are different from classic coiled coils with canonical hydrophobic heptad repeats. Alternatives to the classic knobs-into-holes packing lead to differences in supercoil pitch and diameter that allow water molecules inside the core. This open and more hydrophilic structure does not seem to be destabilized by mutations that would be expected to disrupt classic coiled coils.


==About this Structure==
Tetrameric coiled coil domain of Sendai virus phosphoprotein.,Tarbouriech N, Curran J, Ruigrok RW, Burmeister WP Nat Struct Biol. 2000 Sep;7(9):777-81. PMID:10966649<ref>PMID:10966649</ref>
1EZJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sendai_virus Sendai virus] with EMC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EZJ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Tetrameric coiled coil domain of Sendai virus phosphoprotein., Tarbouriech N, Curran J, Ruigrok RW, Burmeister WP, Nat Struct Biol. 2000 Sep;7(9):777-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10966649 10966649]
</div>
[[Category: RNA-directed RNA polymerase]]
<div class="pdbe-citations 1ezj" style="background-color:#fffaf0;"></div>
[[Category: Sendai virus]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Burmeister, W.P.]]
__TOC__
[[Category: Curran, J.]]
</StructureSection>
[[Category: Ruigrok, R.W.H.]]
[[Category: Large Structures]]
[[Category: Tarbouriech, N.]]
[[Category: Burmeister WP]]
[[Category: CA]]
[[Category: Curran J]]
[[Category: EMC]]
[[Category: Ruigrok RWH]]
[[Category: four stranded coiled coil]]
[[Category: Tarbouriech N]]
[[Category: sendai virus]]
[[Category: tetramer]]
[[Category: viral polymerase]]
 
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