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New page: left|200px<br /><applet load="1f00" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f00, resolution 1.9Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1f00.gif|left|200px]]<br /><applet load="1f00" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1f00, resolution 1.9&Aring;" />
'''CRYSTAL STRUCTURE OF C-TERMINAL 282-RESIDUE FRAGMENT OF ENTEROPATHOGENIC E. COLI INTIMIN'''<br />


==Overview==
==CRYSTAL STRUCTURE OF C-TERMINAL 282-RESIDUE FRAGMENT OF ENTEROPATHOGENIC E. COLI INTIMIN==
Intimin and its translocated intimin receptor (Tir) are bacterial proteins, that mediate adhesion between mammalian cells and attaching and effacing, (A/E) pathogens. Enteropathogenic Escherichia coli (EPEC) causes, significant paediatric morbidity and mortality world-wide. A related A/E, pathogen, enterohaemorrhagic E. coli (EHEC; O157:H7) is one of the most, important food-borne pathogens in North America, Europe and Japan. A, unique and essential feature of A/E bacterial pathogens is the formation, of actin-rich pedestals beneath the intimately adherent bacteria and, localized destruction of the intestinal brush border. The bacterial outer, membrane adhesin, intimin, is necessary for the production of the A/E, lesion and diarrhoea. The A/E bacteria translocate their own receptor for, intimin, Tir, into the membrane of mammalian cells using the type III, secretion system. The translocated Tir triggers additional host signalling, events and actin nucleation, which are essential for lesion formation., Here we describe the the crystal structures of an EPEC intimin, carboxy-terminal fragment alone and in complex with the EPEC Tir, intimin-binding domain, giving insight into the molecular mechanisms of, adhesion of A/E pathogens.
<StructureSection load='1f00' size='340' side='right'caption='[[1f00]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1f00]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F00 FirstGlance]. <br>
1F00 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F00 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f00 OCA], [https://pdbe.org/1f00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f00 RCSB], [https://www.ebi.ac.uk/pdbsum/1f00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f00 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of enteropathogenic Escherichia coli intimin-receptor complex., Luo Y, Frey EA, Pfuetzner RA, Creagh AL, Knoechel DG, Haynes CA, Finlay BB, Strynadka NC, Nature. 2000 Jun 29;405(6790):1073-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10890451 10890451]
== Function ==
[https://www.uniprot.org/uniprot/EAE_ECO27 EAE_ECO27] Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f00_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f00 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Creagh, A.L.]]
[[Category: Creagh AL]]
[[Category: Finlay, B.B.]]
[[Category: Finlay BB]]
[[Category: Frey, E.A.]]
[[Category: Frey EA]]
[[Category: Haynes, C.A.]]
[[Category: Haynes CA]]
[[Category: Knoechel, D.G.]]
[[Category: Knoechel DG]]
[[Category: Luo, Y.]]
[[Category: Luo Y]]
[[Category: Pfuetzner, R.A.]]
[[Category: Pfuetzner RA]]
[[Category: Strynadka, N.C.J.]]
[[Category: Strynadka NCJ]]
[[Category: c-type lectin-like fold]]
[[Category: immunoglobulin-like fold]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:29:05 2007''
Retrieved from "https://proteopedia.org/1f00"