1dl0: Difference between revisions

Latest revision as of 08:23, 6 November 2024

SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C

Structural highlights

1dl0 is a 1 chain structure with sequence from Hadronyche versuta. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TK1C_HADVE This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.

Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge.,Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:https://dx.doi.org/10.1038/75921
↑ Tedford HW, Maggio F, Reenan RA, King G. A model genetic system for testing the in vivo function of peptide toxins. Peptides. 2007 Jan;28(1):51-6. Epub 2006 Dec 1. PMID:17141372 doi:https://dx.doi.org/10.1016/j.peptides.2006.08.026
↑ Gunning SJ, Maggio F, Windley MJ, Valenzuela SM, King GF, Nicholson GM. The Janus-faced atracotoxins are specific blockers of invertebrate K(Ca) channels. FEBS J. 2008 Aug;275(16):4045-59. doi: 10.1111/j.1742-4658.2008.06545.x. Epub, 2008 Jul 9. PMID:18625007 doi:https://dx.doi.org/10.1111/j.1742-4658.2008.06545.x
↑ Wu Y, Cao G, Pavlicek B, Luo X, Nitabach MN. Phase coupling of a circadian neuropeptide with rest/activity rhythms detected using a membrane-tethered spider toxin. PLoS Biol. 2008 Nov 4;6(11):e273. doi: 10.1371/journal.pbio.0060273. PMID:18986214 doi:https://dx.doi.org/10.1371/journal.pbio.0060273
↑ Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:https://dx.doi.org/10.1038/75921

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:https://dx.doi.org/10.1038/75921

[2] Tedford HW, Maggio F, Reenan RA, King G. A model genetic system for testing the in vivo function of peptide toxins. Peptides. 2007 Jan;28(1):51-6. Epub 2006 Dec 1. PMID:17141372 doi:https://dx.doi.org/10.1016/j.peptides.2006.08.026

[3] Gunning SJ, Maggio F, Windley MJ, Valenzuela SM, King GF, Nicholson GM. The Janus-faced atracotoxins are specific blockers of invertebrate K(Ca) channels. FEBS J. 2008 Aug;275(16):4045-59. doi: 10.1111/j.1742-4658.2008.06545.x. Epub, 2008 Jul 9. PMID:18625007 doi:https://dx.doi.org/10.1111/j.1742-4658.2008.06545.x

[4] Wu Y, Cao G, Pavlicek B, Luo X, Nitabach MN. Phase coupling of a circadian neuropeptide with rest/activity rhythms detected using a membrane-tethered spider toxin. PLoS Biol. 2008 Nov 4;6(11):e273. doi: 10.1371/journal.pbio.0060273. PMID:18986214 doi:https://dx.doi.org/10.1371/journal.pbio.0060273

[5] Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:https://dx.doi.org/10.1038/75921

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[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1dl0.png|left|200px]]
-<!--
+==SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C==
-The line below this paragraph, containing "STRUCTURE_1dl0", creates the "Structure Box" on the page.
+<StructureSection load='1dl0' size='340' side='right'caption='[[1dl0]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1dl0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DL0 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl0 OCA], [https://pdbe.org/1dl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dl0 ProSAT]</span></td></tr>
-{{STRUCTURE_1dl0|  PDB=1dl0  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TK1C_HADVE TK1C_HADVE] This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.<ref>PMID:10881200</ref> <ref>PMID:17141372</ref> <ref>PMID:18625007</ref> <ref>PMID:18986214</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.
-===SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C===
+Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge.,Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200<ref>PMID:10881200</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_10881200}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1dl0" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 10881200 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_10881200}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Hadronyche versuta]]
-DL0 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA].
+[[Category: Large Structures]]
+[[Category: King GF]]
-==Reference==
+[[Category: Wang XH]]
-Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10881200 10881200]
-[[Category: Single protein]]
-[[Category: King, G F.]]
-[[Category: Wang, X H.]]
-[[Category: Atracotoxin]]
-[[Category: Cystine knot]]
-[[Category: Insecticidal]]
-[[Category: Neurotoxin]]
-[[Category: Vicinal disulfide]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:13:51 2008''

1dl0: Difference between revisions

Latest revision as of 08:23, 6 November 2024

SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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