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New page: left|200px<br /><applet load="1f32" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f32, resolution 1.75Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1f32.gif|left|200px]]<br /><applet load="1f32" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1f32, resolution 1.75&Aring;" />
'''CRYSTAL STRUCTURE OF ASCARIS PEPSIN INHIBITOR-3'''<br />


==Overview==
==CRYSTAL STRUCTURE OF ASCARIS PEPSIN INHIBITOR-3==
The three-dimensional structures of pepsin inhibitor-3 (PI-3) from Ascaris, suum and of the complex between PI-3 and porcine pepsin at 1. 75 A and, 2.45 A resolution, respectively, have revealed the mechanism of aspartic, protease inhibition by this unique inhibitor. PI-3 has a new fold, consisting of two domains, each comprising an antiparallel beta-sheet, flanked by an alpha-helix. In the enzyme-inhibitor complex, the N-terminal, beta-strand of PI-3 pairs with one strand of the 'active site flap', (residues 70-82) of pepsin, thus forming an eight-stranded beta-sheet that, spans the two proteins. PI-3 has a novel mode of inhibition, using its, N-terminal residues to occupy and therefore block the first three binding, pockets in pepsin for substrate residues C-terminal to the scissile bond, (S1'-S3'). The molecular structure of the pepsin-PI-3 complex suggests new, avenues for the rational design of proteinaceous aspartic proteinase, inhibitors.
<StructureSection load='1f32' size='340' side='right'caption='[[1f32]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1f32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F32 FirstGlance]. <br>
1F32 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F32 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f32 OCA], [https://pdbe.org/1f32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f32 RCSB], [https://www.ebi.ac.uk/pdbsum/1f32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f32 ProSAT]</span></td></tr>
==Reference==
</table>
Structural basis for the inhibition of porcine pepsin by Ascaris pepsin inhibitor-3., Ng KK, Petersen JF, Cherney MM, Garen C, Zalatoris JJ, Rao-Naik C, Dunn BM, Martzen MR, Peanasky RJ, James MN, Nat Struct Biol. 2000 Aug;7(8):653-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10932249 10932249]
== Function ==
[https://www.uniprot.org/uniprot/API3_ASCSU API3_ASCSU] This is an inhibitor of the aspartic protease pepsin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/1f32_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f32 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Ascaris suum]]
[[Category: Ascaris suum]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cherney, M.M.]]
[[Category: Cherney MM]]
[[Category: Garen, C.]]
[[Category: Garen C]]
[[Category: James, M.N.]]
[[Category: James MN]]
[[Category: Ng, K.K.]]
[[Category: Ng KK]]
[[Category: Petersen, J.F.]]
[[Category: Petersen JF]]
[[Category: proteinase inhibitor]]
 
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