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New page: left|200px<br /><applet load="1fan" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fan, resolution 2.0Å" /> '''CREVICE-FORMING MUTAN...
 
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[[Image:1fan.jpg|left|200px]]<br /><applet load="1fan" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fan, resolution 2.0&Aring;" />
'''CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A'''<br />


==Overview==
==CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A==
Crystal structures of four mutants of bovine pancreatic trypsin inhibitor, (F22A, Y23A, N43G, and F45A), engineered to alter their stability, properties, have been determined. The mutated residues, which are highly, conserved among Kunitz-type inhibitors, are located in the rigid core of, the molecule. Replacement of the partially buried bulky residues of the, wild-type protein with smaller residues resulted in crevices open to the, exterior of the molecule. The overall three-dimensional structure of these, mutants is very similar to that of the wild-type protein and only small, rearrangements are observed among the atoms lining the crevices.
<StructureSection load='1fan' size='340' side='right'caption='[[1fan]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fan]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FAN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fan OCA], [https://pdbe.org/1fan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fan RCSB], [https://www.ebi.ac.uk/pdbsum/1fan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fan ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/1fan_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fan ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal structures of four mutants of bovine pancreatic trypsin inhibitor (F22A, Y23A, N43G, and F45A), engineered to alter their stability properties, have been determined. The mutated residues, which are highly conserved among Kunitz-type inhibitors, are located in the rigid core of the molecule. Replacement of the partially buried bulky residues of the wild-type protein with smaller residues resulted in crevices open to the exterior of the molecule. The overall three-dimensional structure of these mutants is very similar to that of the wild-type protein and only small rearrangements are observed among the atoms lining the crevices.


==About this Structure==
Crevice-forming mutants in the rigid core of bovine pancreatic trypsin inhibitor: crystal structures of F22A, Y23A, N43G, and F45A.,Danishefsky AT, Housset D, Kim KS, Tao F, Fuchs J, Woodward C, Wlodawer A Protein Sci. 1993 Apr;2(4):577-87. PMID:8518731<ref>PMID:8518731</ref>
1FAN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FAN OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crevice-forming mutants in the rigid core of bovine pancreatic trypsin inhibitor: crystal structures of F22A, Y23A, N43G, and F45A., Danishefsky AT, Housset D, Kim KS, Tao F, Fuchs J, Woodward C, Wlodawer A, Protein Sci. 1993 Apr;2(4):577-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8518731 8518731]
</div>
<div class="pdbe-citations 1fan" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[BPTI 3D structures|BPTI 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Danishefsky, A.T.]]
[[Category: Danishefsky AT]]
[[Category: Kim, K.S.]]
[[Category: Kim K-S]]
[[Category: Tao, F.]]
[[Category: Tao F]]
[[Category: Wlodawer, A.]]
[[Category: Wlodawer A]]
[[Category: Woodward, C.]]
[[Category: Woodward C]]
[[Category: serine protease inhibitor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:46:33 2007''
Retrieved from "https://proteopedia.org/1fan"