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New page: left|200px<br /><applet load="1fgj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fgj, resolution 2.8Å" /> '''X-RAY STRUCTURE OF HY...
 
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[[Image:1fgj.gif|left|200px]]<br /><applet load="1fgj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fgj, resolution 2.8&Aring;" />
'''X-RAY STRUCTURE OF HYDROXYLAMINE OXIDOREDUCTASE'''<br />


==Overview==
==X-RAY STRUCTURE OF HYDROXYLAMINE OXIDOREDUCTASE==
The 2.8 A crystal structure of hydroxylamine oxidoreductase of a, nitrifying chemoautotrophic bacterium, Nitrosomonas europaea, is, described. Twenty-four haems lie in the centre bottom of the trimeric, molecule, localized in four clusters within each monomer. The haem, clusters within the trimer are aligned to form a ring that has inlet and, outlet sites. The inlet is occupied by a novel haem, P460, and there are, two possible outlet sites per monomer formed by paired haems lying within, a cavity or cleft on the protein surface. The structure suggests pathways, by which electron transfer may occur through the precisely arranged haems, and provides a framework for the interpretation of previous and future, biochemical and genetic observations.
<StructureSection load='1fgj' size='340' side='right'caption='[[1fgj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fgj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FGJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fgj OCA], [https://pdbe.org/1fgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fgj RCSB], [https://www.ebi.ac.uk/pdbsum/1fgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fgj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HAO_NITEU HAO_NITEU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/1fgj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fgj ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 2.8 A crystal structure of hydroxylamine oxidoreductase of a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea, is described. Twenty-four haems lie in the centre bottom of the trimeric molecule, localized in four clusters within each monomer. The haem clusters within the trimer are aligned to form a ring that has inlet and outlet sites. The inlet is occupied by a novel haem, P460, and there are two possible outlet sites per monomer formed by paired haems lying within a cavity or cleft on the protein surface. The structure suggests pathways by which electron transfer may occur through the precisely arranged haems and provides a framework for the interpretation of previous and future biochemical and genetic observations.


==About this Structure==
The 2.8 A structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea.,Igarashi N, Moriyama H, Fujiwara T, Fukumori Y, Tanaka N Nat Struct Biol. 1997 Apr;4(4):276-84. PMID:9095195<ref>PMID:9095195</ref>
1FGJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea] with HEM and HEC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hydroxylamine_oxidase Hydroxylamine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.4 1.7.3.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FGJ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The 2.8 A structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea., Igarashi N, Moriyama H, Fujiwara T, Fukumori Y, Tanaka N, Nat Struct Biol. 1997 Apr;4(4):276-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9095195 9095195]
</div>
[[Category: Hydroxylamine oxidase]]
<div class="pdbe-citations 1fgj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nitrosomonas europaea]]
[[Category: Nitrosomonas europaea]]
[[Category: Single protein]]
[[Category: Igarashi N]]
[[Category: Igarashi, N.]]
[[Category: Moriyama H]]
[[Category: Moriyama, H.]]
[[Category: Tanaka N]]
[[Category: Tanaka, N.]]
[[Category: HEC]]
[[Category: HEM]]
[[Category: nitrification]]
[[Category: oxidoreductase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:54:24 2007''

Latest revision as of 05:56, 13 August 2026

X-RAY STRUCTURE OF HYDROXYLAMINE OXIDOREDUCTASE

1fgj, resolution 2.80Å

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