1eyx: Difference between revisions

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{{Seed}}
[[Image:1eyx.png|left|200px]]


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==CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS==
The line below this paragraph, containing "STRUCTURE_1eyx", creates the "Structure Box" on the page.
<StructureSection load='1eyx' size='340' side='right'caption='[[1eyx]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1eyx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Agarophyton_chilense Agarophyton chilense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene>, <scene name='pdbligand=PUB:PHYCOUROBILIN'>PUB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1eyx|  PDB=1eyx  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyx OCA], [https://pdbe.org/1eyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyx RCSB], [https://www.ebi.ac.uk/pdbsum/1eyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHEA_AGACH PHEA_AGACH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eyx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
R-phycoerythrin, a light-harvesting component from the red algae Gracilaria chilensis, was crystallized by vapour diffusion using ammonium sulfate as precipitant agent. Red crystals grew after one week at 293 K and diffracted to 2.70 A resolution. Three serial macroseeding assays were necessary to grow a second larger crystal to dimensions of 0.68 x 0.16 x 0.16 mm. This crystal diffracted to 2.24 A resolution using synchrotron radiation at beamline BM14 of the European Synchrotron Radiation Facility (ESRF) at Grenoble, France and was used for structure determination. Data were collected at 100 K to a completeness of 98.6%. The crystal was trigonal, space group R3, with unit-cell parameters a = b = 187.3, c = 59.1 A, alpha = beta = 90, gamma = 120 degrees. Data treatment using the CCP4 suite of programs indicated that the crystal was twinned ((I(2))/(I)(2) = 1.41). Molecular replacement was performed with AMoRe using the R-phycoerythrin from Polysiphonia urceolata [Chang et al. (1996), J. Mol. Biol. 249, 424-440] as a search model. In order to overcome the twinning problem, SHELX97 was used for the crystallographic refinement. The twin fraction was 0.48, indicating a nearly perfect hemihedrally twinned crystal. The final R(work) and R(free) factors are 0.16 and 0.25, respectively. All the residues and chromophores of the alpha- and beta-chains are well defined in the electron-density maps. Some residues belonging to the gamma-linker are also recognizable.


===CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS===
Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning.,Contreras-Martel C, Martinez-Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X, Fontecilla-Camps JC Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):52-60. PMID:11134927<ref>PMID:11134927</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_11134927}}, adds the Publication Abstract to the page
<div class="pdbe-citations 1eyx" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 11134927 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_11134927}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Agarophyton chilense]]
1EYX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gracilaria_chilensis Gracilaria chilensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYX OCA].
[[Category: Large Structures]]
 
[[Category: Bunster M]]
==Reference==
[[Category: Contreras-Martel C]]
Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning., Contreras-Martel C, Martinez-Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X, Fontecilla-Camps JC, Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):52-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11134927 11134927]
[[Category: Fontecilla-Camps JC]]
[[Category: Gracilaria chilensis]]
[[Category: Legrand P]]
[[Category: Protein complex]]
[[Category: Martinez-Oyanedel J]]
[[Category: Bunster, M.]]
[[Category: Piras C]]
[[Category: Contreras-Martel, C.]]
[[Category: Vernede X]]
[[Category: Fontecilla-Camps, J C.]]
[[Category: Legrand, P.]]
[[Category: Martinez-Oyanedel, J.]]
[[Category: Piras, C.]]
[[Category: Vernede, X.]]
[[Category: Macroseeding]]
[[Category: Phycobiliprotein]]
[[Category: Protein structure]]
[[Category: R-phycoerythrin]]
[[Category: Red algae]]
[[Category: Sequence]]
[[Category: Twin]]
 
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