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| [[Image:1fpx.jpg|left|200px]]<br /><applet load="1fpx" size="450" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[6cig]] This PDB entry is obsolete and replaced by 6cig |
| caption="1fpx, resolution 1.65Å" />
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| '''CRYSTAL STRUCTURE ANALYSIS OF SELENOMETHIONINE SUBSTITUTED ISOFLAVONE O-METHYLTRANSFERASE'''<br />
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| ==Overview==
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| Chalcone O-methyltransferase (ChOMT) and isoflavone O-methyltransferase, (IOMT) are S-adenosyl-l-methionine (SAM) dependent plant natural product, methyltransferases involved in secondary metabolism in Medicago sativa, (alfalfa). Here we report the crystal structure of ChOMT in complex with, the product S-adenosyl-l-homocysteine and the substrate isoliquiritigenin, (4,2',4'-trihydroxychalcone) refined to 1.8 A as well as the crystal, structure of IOMT in complex with the products S-adenosyl-l-homocysteine, and isoformononetin (4'-hydroxy-7-methoxyisoflavone) refined to 1.4 A., These two OMTs constitute the first plant methyltransferases to be, structurally characterized and reveal a novel oligomerization domain and, the molecular determinants for substrate selection. As such, this work, provides a structural basis for understanding the substrate specificity of, the diverse family of plant OMTs and facilitates the engineering of novel, activities in this extensive class of natural product biosynthetic, enzymes.
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| ==About this Structure==
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| 1FPX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FPX OCA].
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| ==Reference==
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| Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases., Zubieta C, He XZ, Dixon RA, Noel JP, Nat Struct Biol. 2001 Mar;8(3):271-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11224575 11224575]
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| [[Category: Medicago sativa]]
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| [[Category: Single protein]]
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| [[Category: Dixon, R.A.]]
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| [[Category: Noel, J.P.]]
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| [[Category: Zubieta, C.]]
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| [[Category: SAM]]
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| [[Category: s-adenosymethionine complex]]
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| [[Category: selenomethionine]]
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| ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:08:25 2007''
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This PDB entry is obsolete and replaced by 6cig
