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New page: left|200px<br /><applet load="7rsa" size="450" color="white" frame="true" align="right" spinBox="true" caption="7rsa, resolution 1.26Å" /> '''STRUCTURE OF PHOSPHA...
 
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[[Image:7rsa.gif|left|200px]]<br /><applet load="7rsa" size="450" color="white" frame="true" align="right" spinBox="true"
caption="7rsa, resolution 1.26&Aring;" />
'''STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS'''<br />


==Overview==
==STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS==
The structure of phosphate-free bovine ribonuclease A has been refined at, 1.26-A resolution by a restrained least-squares procedure to a final R, factor of 0.15. X-ray diffraction data were collected with an electronic, position-sensitive detector. The final model consists of all atoms in the, polypeptide chain including hydrogens, 188 water sites with full or, partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen, side chains were modeled with two alternate conformations. Major changes, to the active site include the addition of two waters in the, phosphate-binding pocket, disordering of Gln-11, and tilting of the, imidazole ring of His-119. The structure of the protein and of the, associated solvent was extensively compared with three other, high-resolution, refined structures of this enzyme.
<StructureSection load='7rsa' size='340' side='right'caption='[[7rsa]], [[Resolution|resolution]] 1.26&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7rsa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RSA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.26&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsa OCA], [https://pdbe.org/7rsa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rsa RCSB], [https://www.ebi.ac.uk/pdbsum/7rsa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rsa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rs/7rsa_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7rsa ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of phosphate-free bovine ribonuclease A has been refined at 1.26-A resolution by a restrained least-squares procedure to a final R factor of 0.15. X-ray diffraction data were collected with an electronic position-sensitive detector. The final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen side chains were modeled with two alternate conformations. Major changes to the active site include the addition of two waters in the phosphate-binding pocket, disordering of Gln-11, and tilting of the imidazole ring of His-119. The structure of the protein and of the associated solvent was extensively compared with three other high-resolution, refined structures of this enzyme.


==About this Structure==
Structure of phosphate-free ribonuclease A refined at 1.26 A.,Wlodawer A, Svensson LA, Sjolin L, Gilliland GL Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:3401445<ref>PMID:3401445</ref>
7RSA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with TBU and DOD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=7RSA OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of phosphate-free ribonuclease A refined at 1.26 A., Wlodawer A, Svensson LA, Sjolin L, Gilliland GL, Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3401445 3401445]
</div>
<div class="pdbe-citations 7rsa" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Pancreatic ribonuclease]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Gilliland GL]]
[[Category: Gilliland, G.L.]]
[[Category: Wlodawer A]]
[[Category: Wlodawer, A.]]
[[Category: DOD]]
[[Category: TBU]]
[[Category: hydrolase (phosphoric diester)]]
 
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