1fse: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: left|200px<br /><applet load="1fse" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fse, resolution 2.05Å" /> '''CRYSTAL STRUCTURE OF...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1fse.gif|left|200px]]<br /><applet load="1fse" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fse, resolution 2.05&Aring;" />
'''CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS REGULATORY PROTEIN GERE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS REGULATORY PROTEIN GERE==
The small, DNA-binding protein GerE regulates gene transcription in the, terminally differentiated mother-cell compartment during late stages of, sporulation in Bacillus subtilis. This versatile transcription factor, shares sequence homology with the LuxR/FixJ/UhpA family of activators and, modulates the expression of a number of genes, in particular those, encoding the components of the coat that surrounds the mature spore. GerE, orchestrates the final stages of coat deposition and maturation that lead, to a spore with remarkable resistance properties but that must be, responsive to low levels of germination signals. As this germination, process is largely passive and can occur in the absence of de novo protein, synthesis, the correct assembly of germination machinery, including, germinant receptors and energy storage compounds, is crucial to the, survival of the cell. The crystal structure of GerE has been solved at, 2.05 A resolution using multi-wavelength anomalous dispersion techniques, and reveals the nature of the GerE dimer. Each monomer comprises four, alpha-helices, of which the central pair forms a helix-turn-helix, DNA-binding motif. Implications for DNA-binding and the structural, organisation of the LuxR/FixJ/UhpA family of transcription activator, domains are discussed.
<StructureSection load='1fse' size='340' side='right'caption='[[1fse]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1fse]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FSE FirstGlance]. <br>
1FSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FSE OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fse OCA], [https://pdbe.org/1fse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fse RCSB], [https://www.ebi.ac.uk/pdbsum/1fse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fse ProSAT]</span></td></tr>
Crystal structure of GerE, the ultimate transcriptional regulator of spore formation in Bacillus subtilis., Ducros VM, Lewis RJ, Verma CS, Dodson EJ, Leonard G, Turkenburg JP, Murshudov GN, Wilkinson AJ, Brannigan JA, J Mol Biol. 2001 Mar 2;306(4):759-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11243786 11243786]
</table>
== Function ==
[https://www.uniprot.org/uniprot/GERE_BACSU GERE_BACSU] Involved in the regulation of spore formation. Directs the transcription of several genes that encode structural components of the protein coat that encases the mature spore (CotB, CotC, CotG, CotS, CotV, CotW, CotX, CotY and CotZ). Controls also the cgeAB and cgeCDE operons.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/1fse_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fse ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Brannigan, J.A.]]
[[Category: Brannigan JA]]
[[Category: Dodson, E.J.]]
[[Category: Dodson EJ]]
[[Category: Ducros, V.M.A.]]
[[Category: Ducros VM-A]]
[[Category: Leonard, G.]]
[[Category: Leonard G]]
[[Category: Lewis, R.J.]]
[[Category: Lewis RJ]]
[[Category: Murshudov, G.N.]]
[[Category: Murshudov GN]]
[[Category: Turkenburg, J.P.]]
[[Category: Turkenburg JP]]
[[Category: Verma, C.S.]]
[[Category: Verma CS]]
[[Category: Wilkinson, A.J.]]
[[Category: Wilkinson AJ]]
[[Category: GOL]]
[[Category: SO4]]
[[Category: helix-turn-helix dna-binding protein transcriptional regulator]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:14:12 2007''

Latest revision as of 07:18, 7 February 2024

CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS REGULATORY PROTEIN GERE

1fse, resolution 2.05Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1fse&oldid=4043767"