1gwk: Difference between revisions

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{{Seed}}
[[Image:1gwk.png|left|200px]]


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==Carbohydrate binding module family29==
The line below this paragraph, containing "STRUCTURE_1gwk", creates the "Structure Box" on the page.
<StructureSection load='1gwk' size='340' side='right'caption='[[1gwk]], [[Resolution|resolution]] 2.34&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1gwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Piromyces_sp._'equi' Piromyces sp. 'equi']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GWK FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.34&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_1gwk|  PDB=1gwk  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwk OCA], [https://pdbe.org/1gwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gwk RCSB], [https://www.ebi.ac.uk/pdbsum/1gwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gwk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9C171_PIREQ Q9C171_PIREQ]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Carbohydrate-protein recognition is central to many biological processes. Enzymes that act on polysaccharide substrates frequently contain noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target the enzyme to the appropriate substrate. CBMs that recognize specific plant structural polysaccharides are often able to accommodate both the variable backbone and the side-chain decorations of heterogeneous ligands. "CBM29" modules, derived from a noncatalytic component of the Piromyces equi cellulase/hemicellulase complex, provide an example of this selective yet flexible recognition. They discriminate strongly against some polysaccharides while remaining relatively promiscuous toward both beta-1,4-linked manno- and cello-oligosaccharides. This feature may reflect preferential, but flexible, targeting toward glucomannans in the plant cell wall. The three-dimensional structure of CBM29-2 and its complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of the aromatic residues complements the conformation of the target sugar polymer while accommodation of both manno- and gluco-configured oligo- and polysaccharides is conferred by virtue of the plasticity of the direct interactions from their axial and equatorial 2-hydroxyls, respectively. Such flexible ligand recognition targets the anaerobic fungal complex to a range of different components in the plant cell wall and thus plays a pivotal role in the highly efficient degradation of this composite structure by the microbial eukaryote.


===CARBOHYDRATE BINDING MODULE FAMILY29===
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose.,Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:12391332<ref>PMID:12391332</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 12391332 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_12391332}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1GWK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWK OCA].
[[Category: Piromyces sp. 'equi']]
 
[[Category: Charnock SJ]]
==Reference==
[[Category: Davies GJ]]
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12391332 12391332]
[[Category: Nurizzo D]]
[[Category: Piromyces equi]]
[[Category: Single protein]]
[[Category: Charnock, S J.]]
[[Category: Davies, G J.]]
[[Category: Nurizzo, D.]]
[[Category: Carbohydrate binding domain]]
[[Category: Cellohexaose]]
[[Category: Cellulosome]]
[[Category: Glucomannan]]
[[Category: Mannohexaose]]
 
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Retrieved from "https://proteopedia.org/1gwk"