2iu5: Difference between revisions

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New page: left|200px<br /><applet load="2iu5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iu5, resolution 1.60Å" /> '''DIHYDROXYACETONE KIN...
 
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[[Image:2iu5.gif|left|200px]]<br /><applet load="2iu5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2iu5, resolution 1.60&Aring;" />
'''DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS'''<br />


==Overview==
==Dihydroxyacetone kinase operon activator DhaS==
Dihydroxyacetone (Dha) kinases are a novel family of kinases with, signaling and metabolic functions. Here we report the x-ray structures of, the transcriptional activator DhaS and the coactivator DhaQ and, characterize their function. DhaQ is a paralog of the Dha binding Dha, kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional, activator. DhaQ and DhaS form a stable complex that in the presence of Dha, activates transcription of the Lactococcus lactis dha operon. Dha, covalently binds to DhaQ through a hemiaminal bond with a histidine and, thereby induces a conformational change, which is propagated to the, surface via a cantilever-like structure. DhaS binding protects an inverted, repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs, of the operator DNA hypersensitive to DNase I cleavage. The proximal, half-site of the inverted repeat partially overlaps with the predicted -35, consensus sequence of the dha promoter.
<StructureSection load='2iu5' size='340' side='right'caption='[[2iu5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2iu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IU5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iu5 OCA], [https://pdbe.org/2iu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iu5 RCSB], [https://www.ebi.ac.uk/pdbsum/2iu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iu5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHAS_LACLA DHAS_LACLA] In complex with DhaQ, upon activation by dihydroxyacetone, activates transcription of the dhaKLM operon. Binds the inverted repeat sequence 5'-GGACACATN(6)ATTTGTCC-3' located upstream of and partially overlapping with the -35 promoter sequence of the dhaKLM operon promoter.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iu5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iu5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.


==About this Structure==
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.,Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471<ref>PMID:16760471</ref>
2IU5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16760471 16760471]
</div>
[[Category: Lactococcus lactis subsp. lactis il1403]]
<div class="pdbe-citations 2iu5" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Baumann, U.]]
<references/>
[[Category: Christen, S.]]
__TOC__
[[Category: Erni, B.]]
</StructureSection>
[[Category: Srinivas, A.]]
[[Category: Lactococcus lactis subsp. lactis Il1403]]
[[Category: activator]]
[[Category: Large Structures]]
[[Category: dihydroxyacetone]]
[[Category: Baumann U]]
[[Category: lactococcus lactis]]
[[Category: Christen S]]
[[Category: synthase]]
[[Category: Erni B]]
[[Category: tetr family]]
[[Category: Srinivas A]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:32:28 2007''

Latest revision as of 09:29, 9 May 2024

Dihydroxyacetone kinase operon activator DhaS

2iu5, resolution 1.60Å

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