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New page: left|200px<br /><applet load="1g4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g4w, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1g4w.jpg|left|200px]]<br /><applet load="1g4w" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1g4w, resolution 2.2&Aring;" />
'''CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP==
Salmonella spp. utilize a specialized protein secretion system to deliver, a battery of effector proteins into host cells. Several of these effectors, stimulate Cdc42- and Rac1-dependent cytoskeletal changes that promote, bacterial internalization. These potentially cytotoxic alterations are, rapidly reversed by the effector SptP, a tyrosine phosphatase and GTPase, activating protein (GAP) that targets Cdc42 and Rac1. The 2.3 A resolution, crystal structure of an SptP-Rac1 transition state complex reveals an, unusual GAP architecture that mimics host functional homologs. The, phosphatase domain possesses a conserved active site but distinct surface, properties. Binding to Rac1 induces a dramatic stabilization in SptP of a, four-helix bundle that makes extensive contacts with the Switch I and, Switch II regions of the GTPase.
<StructureSection load='1g4w' size='340' side='right'caption='[[1g4w]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1g4w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G4W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g4w OCA], [https://pdbe.org/1g4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g4w RCSB], [https://www.ebi.ac.uk/pdbsum/1g4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g4w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPTP_SALTY SPTP_SALTY] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein includes tyrosine phosphatase and GTPase activating protein (GAP) activities. After bacterial internalization, GAP mediates the reversal of the cytoskeletal changes induced by SopE. This function is independent of its tyrosine phosphatase activity, which remains unclear.<ref>PMID:8866485</ref> <ref>PMID:10499590</ref> <ref>PMID:11163217</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g4/1g4w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g4w ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1G4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G4W OCA].
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
 
== References ==
==Reference==
<references/>
Modulation of host signaling by a bacterial mimic: structure of the Salmonella effector SptP bound to Rac1., Stebbins CE, Galan JE, Mol Cell. 2000 Dec;6(6):1449-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11163217 11163217]
__TOC__
[[Category: Salmonella typhimurium]]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Galan, J.E.]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Stebbins, C.E.]]
[[Category: Galan JE]]
[[Category: 4-helix bundle]]
[[Category: Stebbins CE]]
[[Category: disorder]]
[[Category: gtpase activating protein]]
[[Category: tyrosine phosphatase]]
[[Category: virulence factor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:42:31 2007''

Latest revision as of 07:22, 7 February 2024

CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP

1g4w, resolution 2.20Å

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