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New page: left|200px<br /><applet load="1gbg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gbg, resolution 1.8Å" /> '''BACILLUS LICHENIFORMI...
 
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[[Image:1gbg.gif|left|200px]]<br /><applet load="1gbg" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gbg, resolution 1.8&Aring;" />
'''BACILLUS LICHENIFORMIS BETA-GLUCANASE'''<br />


==Overview==
==BACILLUS LICHENIFORMIS BETA-GLUCANASE==
The crystal structure of the 1,3-1,4-beta-D-glucan 4-glucanohydrolase from, Bacillus licheniformis is solved at a resolution of 1.8 A and refined to R, = 16.5%. The protein has a similar beta-sandwich structure as the, homologous enzyme from Bacillus macerans and the hybrid H(A16-M). This, demonstrates that the jellyroll fold of these proteins is remarkably rigid, and only weakly influenced by crystal contacts. The crystal structure, permits to extend mechanistic considerations derived for the B., licheniformis enzyme to the entire class of bacterial, 1,3-1,4-beta-D-glucan 4-glucanohydrolases.
<StructureSection load='1gbg' size='340' side='right'caption='[[1gbg]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GBG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gbg OCA], [https://pdbe.org/1gbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gbg RCSB], [https://www.ebi.ac.uk/pdbsum/1gbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gbg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GUB_BACLI GUB_BACLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/1gbg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gbg ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the 1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis is solved at a resolution of 1.8 A and refined to R = 16.5%. The protein has a similar beta-sandwich structure as the homologous enzyme from Bacillus macerans and the hybrid H(A16-M). This demonstrates that the jellyroll fold of these proteins is remarkably rigid and only weakly influenced by crystal contacts. The crystal structure permits to extend mechanistic considerations derived for the B. licheniformis enzyme to the entire class of bacterial 1,3-1,4-beta-D-glucan 4-glucanohydrolases.


==About this Structure==
Crystal structure of Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase at 1.8 A resolution.,Hahn M, Pons J, Planas A, Querol E, Heinemann U FEBS Lett. 1995 Oct 30;374(2):221-4. PMID:7589539<ref>PMID:7589539</ref>
1GBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GBG OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase at 1.8 A resolution., Hahn M, Pons J, Planas A, Querol E, Heinemann U, FEBS Lett. 1995 Oct 30;374(2):221-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7589539 7589539]
</div>
<div class="pdbe-citations 1gbg" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
[[Category: Licheninase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Hahn M]]
[[Category: Hahn, M.]]
[[Category: Heinemann U]]
[[Category: Heinemann, U.]]
[[Category: CA]]
[[Category: hydrolase (glucanase)]]
 
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