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New page: left|200px<br /><applet load="1glu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1glu, resolution 2.900Å" /> '''CRYSTALLOGRAPHIC AN...
 
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[[Image:1glu.jpg|left|200px]]<br /><applet load="1glu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1glu, resolution 2.900&Aring;" />
'''CRYSTALLOGRAPHIC ANALYSIS OF THE INTERACTION OF THE GLUCOCORTICOID RECEPTOR WITH DNA'''<br />


==Overview==
==CRYSTALLOGRAPHIC ANALYSIS OF THE INTERACTION OF THE GLUCOCORTICOID RECEPTOR WITH DNA==
Two crystal structures of the glucocorticoid receptor DNA-binding domain, complexed with DNA are reported. The domain has a globular fold which, contains two Zn-nucleated substructures of distinct conformation and, function. When it binds DNA, the domain dimerizes, placing the subunits in, adjacent major grooves. In one complex, the DNA has the symmetrical, consensus target sequence; in the second, the central spacing between the, target's half-sites is larger by one base pair. This results in one, subunit interacting specifically with the consensus target half-site and, the other nonspecifically with a noncognate element. The DNA-induced dimer, fixes the separation of the subunits' recognition surfaces so that the, spacing between the half-sites becomes a critical feature of the target, sequence's identity.
<StructureSection load='1glu' size='340' side='right'caption='[[1glu]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1glu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GLU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GLU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1glu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1glu OCA], [https://pdbe.org/1glu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1glu RCSB], [https://www.ebi.ac.uk/pdbsum/1glu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1glu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCR_RAT GCR_RAT] Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation (By similarity).<ref>PMID:12917342</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/1glu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1glu ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1GLU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GLU OCA].
*[[Glucocorticoid receptor 3D structures|Glucocorticoid receptor 3D structures]]
 
== References ==
==Reference==
<references/>
Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA., Luisi BF, Xu WX, Otwinowski Z, Freedman LP, Yamamoto KR, Sigler PB, Nature. 1991 Aug 8;352(6335):497-505. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1865905 1865905]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Freedman LP]]
[[Category: Freedman, L.P.]]
[[Category: Luisi BF]]
[[Category: Luisi, B.F.]]
[[Category: Otwinowski Z]]
[[Category: Otwinowski, Z.]]
[[Category: Sigler PB]]
[[Category: Sigler, P.B.]]
[[Category: Xu WX]]
[[Category: Xu, W.X.]]
[[Category: Yamamoto KR]]
[[Category: Yamamoto, K.R.]]
[[Category: ZN]]
[[Category: double helix]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:09:39 2007''

Latest revision as of 07:25, 7 February 2024

CRYSTALLOGRAPHIC ANALYSIS OF THE INTERACTION OF THE GLUCOCORTICOID RECEPTOR WITH DNA

1glu, resolution 2.90Å

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