1hk6: Difference between revisions
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New page: left|200px<br /><applet load="1hk6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hk6" /> '''RAL BINDING DOMAIN FROM SEC5'''<br /> ==Ove... |
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== | ==Ral binding domain from Sec5== | ||
The exocyst complex is involved in the final stages of exocytosis, when | <StructureSection load='1hk6' size='340' side='right'caption='[[1hk6]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1hk6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HK6 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hk6 OCA], [https://pdbe.org/1hk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hk6 RCSB], [https://www.ebi.ac.uk/pdbsum/1hk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hk6 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/EXOC2_MOUSE EXOC2_MOUSE] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.[UniProtKB:Q96KP1] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hk6_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hk6 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The exocyst complex is involved in the final stages of exocytosis, when vesicles are targeted to the plasma membrane and dock. The regulation of exocytosis is vital for a number of processes, for example, cell polarity, embryogenesis, and neuronal growth formation. Regulation of the exocyst complex in mammals was recently shown to be dependent upon binding of the small G protein, Ral, to Sec5, a central component of the exocyst. This interaction is thought to be necessary for anchoring the exocyst to secretory vesicles. We have determined the structure of the Ral-binding domain of Sec5 and shown that it adopts a fold that has not been observed in a G protein effector before. This fold belongs to the immunoglobulin superfamily in a subclass known as IPT domains. We have mapped the Ral binding site on this domain and found that it overlaps with protein-protein interaction sites on other IPT domains but that it is completely different from the G protein-geranyl-geranyl interaction face of the Ig-like domain of the Rho guanine nucleotide dissociation inhibitor. This mapping, along with available site-directed mutagenesis data, allows us to predict how Ral and Sec5 may interact. | |||
Structure of the GTPase-binding domain of Sec5 and elucidation of its Ral binding site.,Mott HR, Nietlispach D, Hopkins LJ, Mirey G, Camonis JH, Owen D J Biol Chem. 2003 May 9;278(19):17053-9. Epub 2003 Mar 6. PMID:12624092<ref>PMID:12624092</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1hk6" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Camonis JH]] | |||
[[Category: Camonis | [[Category: Hopkins LJ]] | ||
[[Category: Hopkins | [[Category: Mirey G]] | ||
[[Category: Mirey | [[Category: Mott HR]] | ||
[[Category: Mott | [[Category: Nietlispach D]] | ||
[[Category: Nietlispach | [[Category: Owen D]] | ||
[[Category: Owen | |||
Latest revision as of 05:31, 15 May 2024
Ral binding domain from Sec5
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