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{{Seed}}
[[Image:1kbm.png|left|200px]]


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==SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE THYMINE==
The line below this paragraph, containing "STRUCTURE_1kbm", creates the "Structure Box" on the page.
<StructureSection load='1kbm' size='340' side='right'caption='[[1kbm]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1kbm]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KBM FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=S6G:6-THIO-2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>S6G</scene></td></tr>
{{STRUCTURE_1kbm|  PDB=1kbm  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kbm OCA], [https://pdbe.org/1kbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kbm RCSB], [https://www.ebi.ac.uk/pdbsum/1kbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kbm ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The incorporation of 6-thioguanine (S6G) into DNA is an essential step in the cytotoxic activity of thiopurines. However, the structural effects of this substitution on duplex DNA have not been fully characterized. Here, we present the solution structures of DNA duplexes containing S6G opposite thymine (S6G.T) and opposite cytosine (S6G.C), solved by high-resolution NMR spectroscopy and restrained molecular dynamics. The data indicate that both duplexes adopt right-handed helical conformations with all Watson-Crick hydrogen bonding in place. The S6G.T structures exhibit a wobble-type base pairing at the lesion site, with thymine shifted toward the major groove and S6G displaced toward the minor groove. Aside from the lesion site, the helices, including the flanking base pairs, are not highly perturbed by the presence of the lesion. Surprisingly, thermal dependence experiments suggest greater stability in the S6G-T mismatch than the S6G-C base pair.


===SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE THYMINE===
Structural effect of the anticancer agent 6-thioguanine on duplex DNA.,Bohon J, de los Santos CR Nucleic Acids Res. 2003 Feb 15;31(4):1331-8. PMID:12582253<ref>PMID:12582253</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1kbm" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 12582253 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_12582253}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBM OCA].
[[Category: Bohon J]]
 
[[Category: De Los Santos CR]]
==Reference==
Structural effect of the anticancer agent 6-thioguanine on duplex DNA., Bohon J, de los Santos CR, Nucleic Acids Res. 2003 Feb 15;31(4):1331-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12582253 12582253]
[[Category: Bohon, J.]]
[[Category: Santos, C R.De Los.]]
[[Category: 6-thioguanine]]
[[Category: 6tg]]
[[Category: B-form dna]]
[[Category: Double helix]]
[[Category: S6g]]
[[Category: Tg]]
[[Category: Thioguanine]]
[[Category: Thiopurine]]
 
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