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New page: left|200px<br /><applet load="1i1h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i1h, resolution 2.60Å" /> '''CRYSTAL STRUCTURE AN...
 
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[[Image:1i1h.gif|left|200px]]<br /><applet load="1i1h" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1i1h, resolution 2.60&Aring;" />
'''CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID'''<br />


==Overview==
==CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID==
BACKGROUND: The crystal structure of precorrin-8x methyl mutase (CobH), an, enzyme of the aerobic pathway to vitamin B12, provides evidence that the, mechanism for methyl migration can plausibly be regarded as an allowed, [1,5]-sigmatropic shift of a methyl group from C-11 to C-12 at the C ring, of precorrin-8x to afford hydrogenobyrinic acid. RESULTS: The dimeric, structure of CobH creates a set of shared active sites that readily, discriminate between different tautomers of precorrin-8x and select a, discrete tautomer for sigmatropic rearrangement. The active site contains, a strictly conserved histidine residue close to the site of methyl, migration in ring C of the substrate. CONCLUSION: Analysis of the, structure with bound product suggests that the [1,5]-sigmatropic shift, proceeds by protonation of the ring C nitrogen, leading to subsequent, methyl migration.
<StructureSection load='1i1h' size='340' side='right'caption='[[1i1h]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1i1h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_denitrificans_(nom._rej.) Pseudomonas denitrificans (nom. rej.)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I1H FirstGlance]. <br>
1I1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_denitrificans Pseudomonas denitrificans] with COJ as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Precorrin-8X_methylmutase Precorrin-8X methylmutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.1.2 5.4.1.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I1H OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COJ:HYDROGENOBYRINIC+ACID'>COJ</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i1h OCA], [https://pdbe.org/1i1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i1h RCSB], [https://www.ebi.ac.uk/pdbsum/1i1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i1h ProSAT]</span></td></tr>
Crystal structure of precorrin-8x methyl mutase., Shipman LW, Li D, Roessner CA, Scott AI, Sacchettini JC, Structure. 2001 Jul 3;9(7):587-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11470433 11470433]
</table>
[[Category: Precorrin-8X methylmutase]]
== Function ==
[[Category: Pseudomonas denitrificans]]
[https://www.uniprot.org/uniprot/COBH_SINSX COBH_SINSX] Catalyzes the conversion of precorrin-8X to hydrogenobyrinate.<ref>PMID:1732194</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Li, D.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Roessner, C.A.]]
Check<jmol>
[[Category: Sacchettini, J.C.]]
  <jmolCheckbox>
[[Category: Scott, A.I.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/1i1h_consurf.spt"</scriptWhenChecked>
[[Category: Shipman, L.W.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: COJ]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: precorrin]]
  </jmolCheckbox>
[[Category: vitamin b12]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i1h ConSurf].
 
<div style="clear:both"></div>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:58:27 2007''
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Li D]]
[[Category: Roessner CA]]
[[Category: Sacchettini JC]]
[[Category: Scott AI]]
[[Category: Shipman LW]]

Latest revision as of 07:31, 7 February 2024

CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID

1i1h, resolution 2.60Å

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