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New page: left|200px<br /><applet load="1iaw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iaw, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1iaw.gif|left|200px]]<br /><applet load="1iaw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1iaw, resolution 2.4&Aring;" />
'''CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA'''<br />


==Overview==
==CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA==
NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase, activities when a Lys residue is substituted for Leu 43. The NaeI-DNA, structure demonstrates that each of the two domains of NaeI recognizes one, molecule of DNA duplex. DNA recognition induces dramatic rearrangements:, narrowing the binding site of the Topo domain 16 A to grip DNA, widening, that of the Endo domain 8 A to encircle and bend DNA 45 degrees for, cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA, structure presents the first example of novel recognition of two copies of, one DNA sequence by two different amino acid sequences and two different, structural motifs in one polypeptide.
<StructureSection load='1iaw' size='340' side='right'caption='[[1iaw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1iaw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IAW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iaw OCA], [https://pdbe.org/1iaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iaw RCSB], [https://www.ebi.ac.uk/pdbsum/1iaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iaw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref>


==About this Structure==
==See Also==
1IAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
== References ==
==Reference==
<references/>
Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement., Huai Q, Colandene JD, Topal MD, Ke H, Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11473254 11473254]
__TOC__
[[Category: Lechevalieria aerocolonigenes]]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Lentzea aerocolonigenes]]
[[Category: Colandene, J.D.]]
[[Category: Colandene JD]]
[[Category: Huai, Q.]]
[[Category: Huai Q]]
[[Category: Ke, H.]]
[[Category: Ke H]]
[[Category: Topal, M.D.]]
[[Category: Topal MD]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:13:34 2007''

Latest revision as of 07:33, 7 February 2024

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

1iaw, resolution 2.40Å

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