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New page: left|200px<br /><applet load="1if0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1if0, resolution 12.Å" /> '''PSEUDO-ATOMIC MODEL O...
 
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[[Image:1if0.gif|left|200px]]<br /><applet load="1if0" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1if0, resolution 12.&Aring;" />
'''PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)'''<br />


==Overview==
==PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)==
Large-scale conformational changes transform viral precursors into, infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked, topology. We have visualized its precursor, Prohead-II, by cryoelectron, microscopy and modeled the conformational change by appropriately adapting, Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching, to an entirely different set of interactions; in addition, two motifs, undergo refolding. These changes stabilize the capsid by increasing the, surface area buried at interfaces and bringing the cross-link-forming, residues, initially approximately 40 angstroms apart, close together. The, inner surface of Prohead-II is negatively charged, suggesting that the, transition is triggered electrostatically by DNA packaging.
<SX load='1if0' size='340' side='right' viewer='molstar' caption='[[1if0]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1if0]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IF0 FirstGlance]. <br>
1IF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1if0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1if0 OCA], [https://pdbe.org/1if0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1if0 RCSB], [https://www.ebi.ac.uk/pdbsum/1if0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1if0 ProSAT]</span></td></tr>
==Reference==
</table>
Virus maturation involving large subunit rotations and local refolding., Conway JF, Wikoff WR, Cheng N, Duda RL, Hendrix RW, Johnson JE, Steven AC, Science. 2001 Apr 27;292(5517):744-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11326105 11326105]
== Function ==
[[Category: Enterobacteria phage hk620]]
[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Cheng, N.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Conway, J.F.]]
Check<jmol>
[[Category: Duda, R.L.]]
  <jmolCheckbox>
[[Category: Hendrix, R.W.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/1if0_consurf.spt"</scriptWhenChecked>
[[Category: Johnson, J.E.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Steven, A.C.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Wikoff, W.R.]]
  </jmolCheckbox>
[[Category: bacteriophage]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1if0 ConSurf].
[[Category: capsid]]
<div style="clear:both"></div>
[[Category: cryoem]]
== References ==
[[Category: icosahedral virus]]
<references/>
[[Category: pseudo-atomic model.]]
__TOC__
[[Category: virus]]
</SX>
 
[[Category: Escherichia virus HK97]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:21:15 2007''
[[Category: Large Structures]]
[[Category: Cheng N]]
[[Category: Conway JF]]
[[Category: Duda RL]]
[[Category: Hendrix RW]]
[[Category: Johnson JE]]
[[Category: Steven AC]]
[[Category: Wikoff WR]]
Retrieved from "https://proteopedia.org/1if0"