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New page: left|200px<br /><applet load="1ij5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ij5, resolution 3.0Å" /> '''METAL-FREE STRUCTURE ...
 
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[[Image:1ij5.gif|left|200px]]<br /><applet load="1ij5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ij5, resolution 3.0&Aring;" />
'''METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD'''<br />


==Overview==
==METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD==
Acellular slime mold, Physarum polycephalum, has a unique wound-healing, system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large, aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N, terminus by a proteinase in plasmodia (CBP40delta), which does not, aggregate in the Ca(2+)-bound form. Here we report the crystal structures, of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both, structures consist of three domains: coiled-coil, intervening, and, EF-hand. The topology of the EF-hand domain is similar to that of calpain., The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands, through a large hydrophobic interface, necessary for high Ca(2+) affinity., Conformational change upon Ca(2+) binding is small; however, the structure, of CBP40delta provides novel insights into the mechanism of, Ca(2+)-dependent oligomerization.
<StructureSection load='1ij5' size='340' side='right'caption='[[1ij5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ij5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IJ5 FirstGlance]. <br>
1IJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IJ5 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ij5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ij5 OCA], [https://pdbe.org/1ij5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ij5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ij5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ij5 ProSAT]</span></td></tr>
==Reference==
</table>
Metal-free and Ca2+-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum., Iwasaki W, Sasaki H, Nakamura A, Kohama K, Tanokura M, Structure. 2003 Jan;11(1):75-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12517342 12517342]
== Function ==
[https://www.uniprot.org/uniprot/LAV1_PHYPO LAV1_PHYPO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ij/1ij5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ij5 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Physarum polycephalum]]
[[Category: Physarum polycephalum]]
[[Category: Single protein]]
[[Category: Iwasaki W]]
[[Category: Iwasaki, W.]]
[[Category: Kohama K]]
[[Category: Kohama, K.]]
[[Category: Nakamura A]]
[[Category: Nakamura, A.]]
[[Category: Sasaki H]]
[[Category: Sasaki, H.]]
[[Category: Tanokura M]]
[[Category: Tanokura, M.]]
[[Category: cbp40]]
[[Category: fourty kda calcium binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:26:28 2007''

Latest revision as of 13:24, 13 March 2024

METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD

1ij5, resolution 3.00Å

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