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New page: left|200px<br /><applet load="1ini" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ini, resolution 1.82Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1ini.jpg|left|200px]]<br /><applet load="1ini" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ini, resolution 1.82&Aring;" />
'''CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHETASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS, COMPLEXED WITH CDP-ME AND MG2+'''<br />


==Overview==
==CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHETASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS, COMPLEXED WITH CDP-ME AND MG2+==
The YgbP protein of Escherichia coli encodes the enzyme, 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) synthetase, a member of, the cytidyltransferase family of enzymes. CDP-ME is an intermediate in the, mevalonate-independent pathway for isoprenoid biosynthesis in a number of, prokaryotic organisms, algae, the plant plastids and the malaria parasite., Because vertebrates synthesize isoprenoid precursors using a mevalonate, pathway, CDP-ME synthetase and other enzymes of the mevalonate-independent, pathway for isoprenoid production represent attractive targets for the, structure-based design of selective antibacterial, herbicidal and, antimalarial drugs. The high-resolution structures of E. coli CDP-ME, synthetase in the apo form and complexed with both CTP-Mg2+ and, CDP-ME-Mg2+ reveal the stereochemical principles underlying both substrate, and product recognition as well as catalysis in CDP-ME synthetase., Moreover, these complexes represent the first experimental structures for, any cytidyltransferase with both substrates and products bound.
<StructureSection load='1ini' size='340' side='right'caption='[[1ini]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ini]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1INI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1INI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDM:4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL'>CDM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ini FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ini OCA], [https://pdbe.org/1ini PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ini RCSB], [https://www.ebi.ac.uk/pdbsum/1ini PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ini ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ISPD_ECOLI ISPD_ECOLI] Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).[HAMAP-Rule:MF_00108]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/1ini_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ini ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The YgbP protein of Escherichia coli encodes the enzyme 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) synthetase, a member of the cytidyltransferase family of enzymes. CDP-ME is an intermediate in the mevalonate-independent pathway for isoprenoid biosynthesis in a number of prokaryotic organisms, algae, the plant plastids and the malaria parasite. Because vertebrates synthesize isoprenoid precursors using a mevalonate pathway, CDP-ME synthetase and other enzymes of the mevalonate-independent pathway for isoprenoid production represent attractive targets for the structure-based design of selective antibacterial, herbicidal and antimalarial drugs. The high-resolution structures of E. coli CDP-ME synthetase in the apo form and complexed with both CTP-Mg2+ and CDP-ME-Mg2+ reveal the stereochemical principles underlying both substrate and product recognition as well as catalysis in CDP-ME synthetase. Moreover, these complexes represent the first experimental structures for any cytidyltransferase with both substrates and products bound.


==About this Structure==
Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis.,Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP Nat Struct Biol. 2001 Jul;8(7):641-8. PMID:11427897<ref>PMID:11427897</ref>
1INI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and CDM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1INI OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis., Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP, Nat Struct Biol. 2001 Jul;8(7):641-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11427897 11427897]
</div>
[[Category: Escherichia coli]]
<div class="pdbe-citations 1ini" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
[[Category: Bowman, M.E.]]
[[Category: Cane, D.E.]]
[[Category: Chow, C.]]
[[Category: Kang, I.]]
[[Category: Kwiatkowski, W.]]
[[Category: Lillo, A.]]
[[Category: Noel, J.P.]]
[[Category: Richard, S.B.]]
[[Category: CDM]]
[[Category: MG]]
[[Category: cdpme]]
[[Category: cytidylyltransferase]]
[[Category: deoxyxylulose-5-phosphate pathway (dxp)]]
[[Category: isoprenoid biosynthesis]]
[[Category: mg]]
[[Category: ygbp]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:31:18 2007''
==See Also==
*[[MEP cytidylyltransferase 3D structures|MEP cytidylyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Bowman ME]]
[[Category: Cane DE]]
[[Category: Chow C]]
[[Category: Kang I]]
[[Category: Kwiatkowski W]]
[[Category: Lillo A]]
[[Category: Noel JP]]
[[Category: Richard SB]]
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