6ebx: Difference between revisions

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{{Seed}}
[[Image:6ebx.png|left|200px]]


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==STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION==
The line below this paragraph, containing "STRUCTURE_6ebx", creates the "Structure Box" on the page.
<StructureSection load='6ebx' size='340' side='right'caption='[[6ebx]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[6ebx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EBX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
{{STRUCTURE_6ebx|  PDB=6ebx  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ebx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebx OCA], [https://pdbe.org/6ebx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ebx RCSB], [https://www.ebi.ac.uk/pdbsum/6ebx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/3S1EB_LATSE 3S1EB_LATSE] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) (tested on Torpedo marmorata, Kd=0.07 nM), and with low affinity to neuronal alpha-7/CHRNA7 nAChRs (tested on chimeric alpha-7/CHRNA7, Kd=22 uM) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission (PubMed:7721859). Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site.<ref>PMID:9305882</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/6ebx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=6ebx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four disulfide bridges, was crystallized in a new orthorhombic form by using thiocyanate as crystallizing agent. The space group is P2(1)2(1)2(1) with a = 53.36 (4), b = 40.89 (4), c = 55.71 (5) A, V = 121533.1 A and Z = 8. X-ray diffraction data were recorded at the LURE synchrotron facility (lambda = 1.405 A). The structure was solved by molecular replacement and shows a dimeric association through an anti-parallel beta-sheet around the twofold non-crystallographic axis. The two independent molecules, one SCN- ion and 97 associated water molecules were refined by molecular dynamics and annealing techniques to R = 19.6% (10,913 Fobs, resolution 5-1.7 A). The thiocyanate ion is located at the interface of the dimer and close to the non-crystallographic twofold axis.


===STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION===
Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution.,Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:1418823<ref>PMID:1418823</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_1418823}}, adds the Publication Abstract to the page
<div class="pdbe-citations 6ebx" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 1418823 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_1418823}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
6EBX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBX OCA].
 
==Reference==
Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution., Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A, Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1418823 1418823]
[[Category: Laticauda semifasciata]]
[[Category: Laticauda semifasciata]]
[[Category: Single protein]]
[[Category: Prange T]]
[[Category: Prange, T.]]
[[Category: Saludjian P]]
[[Category: Saludjian, P.]]
[[Category: Toxin]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul  3 14:17:31 2008''

Latest revision as of 09:52, 23 October 2024

STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION

6ebx, resolution 1.70Å

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