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New page: left|200px<br /><applet load="1j3r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j3r, resolution 2.18Å" /> '''Crystal structure of...
 
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[[Image:1j3r.jpg|left|200px]]<br /><applet load="1j3r" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1j3r, resolution 2.18&Aring;" />
'''Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate'''<br />


==Overview==
==Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate==
The gene encoding phosphoglucose isomerase was cloned from Thermococcus, litoralis, and functionally expressed in Escherichia coli. The purified, enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized., The inhibition constants for four competitive inhibitors were determined., The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity, was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA., Enzymes with mutations in conserved histidine and glutamate residues in, their cupin motifs contained no metals, and showed large decreases in, k(cat). The circular dichroism spectra of the mutant enzymes and the wild, type enzyme were essentially the same but with slight differences.
<StructureSection load='1j3r' size='340' side='right'caption='[[1j3r]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1j3r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J3R FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6PG:6-PHOSPHOGLUCONIC+ACID'>6PG</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3r OCA], [https://pdbe.org/1j3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j3r RCSB], [https://www.ebi.ac.uk/pdbsum/1j3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j3r ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G6PI_THELI G6PI_THELI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j3/1j3r_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j3r ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1J3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis] with 6PG and FE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J3R OCA].
*[[Phosphoglucose isomerase 3D structures|Phosphoglucose isomerase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis., Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T, FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12560104 12560104]
[[Category: Large Structures]]
[[Category: Glucose-6-phosphate isomerase]]
[[Category: Single protein]]
[[Category: Thermococcus litoralis]]
[[Category: Thermococcus litoralis]]
[[Category: Fushinobu, S.]]
[[Category: Fushinobu S]]
[[Category: Hidaka, M.]]
[[Category: Hidaka M]]
[[Category: Ito, S.]]
[[Category: Ito S]]
[[Category: Jeong, J.J.]]
[[Category: Jeong J-J]]
[[Category: Shoun, H.]]
[[Category: Shoun H]]
[[Category: Wakagi, T.]]
[[Category: Wakagi T]]
[[Category: 6PG]]
[[Category: FE]]
[[Category: archaea]]
[[Category: cupin superfamily]]
[[Category: gluconate 6-phosphate]]
[[Category: iron ion]]
[[Category: phosphoglucose isomerase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:55:08 2007''

Latest revision as of 07:14, 25 October 2023

Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate

1j3r, resolution 2.18Å

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