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New page: left|200px<br /><applet load="1jdl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jdl, resolution 1.7Å" /> '''Structure of cytochro...
 
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[[Image:1jdl.jpg|left|200px]]<br /><applet load="1jdl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1jdl, resolution 1.7&Aring;" />
'''Structure of cytochrome c2 from Rhodospirillum Centenum'''<br />


==Overview==
==Structure of cytochrome c2 from Rhodospirillum Centenum==
Cytochrome c(2) from the purple photosynthetic bacterium Rhodospirillum, centenum has been crystallized by the sitting-drop vapour-diffusion, method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 29.7, b = 59.9, c = 65.4 A, and diffract to, a resolution limit of 1.7 A. The Fe-atom position was determined from its, anomalous scattering contribution and a molecular-replacement solution was, calculated. The correctness of the solution was confirmed by parallel, isomorphous replacement studies. The resulting model has a type I, cytochrome fold with two features, an extended alpha-helix and a, surface-charge distribution, that are distinctive to this protein. The, implications of these structural features for the ability of the, cytochrome to serve as an electron carrier are discussed.
<StructureSection load='1jdl' size='340' side='right'caption='[[1jdl]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jdl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillum_centenum Rhodospirillum centenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JDL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jdl OCA], [https://pdbe.org/1jdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jdl RCSB], [https://www.ebi.ac.uk/pdbsum/1jdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jdl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CYC22_RHOCE CYC22_RHOCE] Cytochrome c2 is found mainly in purple, non-sulfur, photosynthetic bacteria where it functions as the electron donor to the oxidized bacteriochlorophyll in the photophosphorylation pathway. However, it may also have a role in the respiratory chain and is found in some non-photosynthetic bacteria.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jd/1jdl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jdl ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytochrome c(2) from the purple photosynthetic bacterium Rhodospirillum centenum has been crystallized by the sitting-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 29.7, b = 59.9, c = 65.4 A, and diffract to a resolution limit of 1.7 A. The Fe-atom position was determined from its anomalous scattering contribution and a molecular-replacement solution was calculated. The correctness of the solution was confirmed by parallel isomorphous replacement studies. The resulting model has a type I cytochrome fold with two features, an extended alpha-helix and a surface-charge distribution, that are distinctive to this protein. The implications of these structural features for the ability of the cytochrome to serve as an electron carrier are discussed.


==About this Structure==
Structure of cytochrome c2 from Rhodospirillum centenum.,Camara-Artigas A, Williams JC, Allen JP Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1498-505. Epub, 2001 Oct 25. PMID:11679712<ref>PMID:11679712</ref>
1JDL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_centenum Rhodospirillum centenum] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JDL OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of cytochrome c2 from Rhodospirillum centenum., Camara-Artigas A, Williams JC, Allen JP, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1498-505. Epub, 2001 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11679712 11679712]
</div>
<div class="pdbe-citations 1jdl" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodospirillum centenum]]
[[Category: Rhodospirillum centenum]]
[[Category: Single protein]]
[[Category: Allen JP]]
[[Category: Allen, J.P.]]
[[Category: Camara-Artigas A]]
[[Category: Camara-Artigas, A.]]
[[Category: Williams JC]]
[[Category: Williams, J.C.]]
[[Category: HEM]]
[[Category: alpha helix]]
 
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Latest revision as of 06:16, 13 August 2026

Structure of cytochrome c2 from Rhodospirillum Centenum

1jdl, resolution 1.70Å

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