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New page: left|200px<br /><applet load="1jgt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jgt, resolution 1.95Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1jgt.gif|left|200px]]<br /><applet load="1jgt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1jgt, resolution 1.95&Aring;" />
'''CRYSTAL STRUCTURE OF BETA-LACTAM SYNTHETASE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF BETA-LACTAM SYNTHETASE==
The enzyme beta-lactam synthetase (beta-LS) catalyzes the formation of the, beta-lactam ring in clavulanic acid, a clinically important beta-lactamase, inhibitor. Whereas the penicillin beta-lactam ring is generated by, isopenicillin N synthase (IPNS) in the presence of ferrous ion and, dioxygen, beta-LS uses ATP and Mg2+ as cofactors. According to sequence, alignments, beta-LS is homologous to class B asparagine synthetases, (AS-Bs), ATP/Mg2+-dependent enzymes that convert aspartic acid to, asparagine. Here we report the first crystal structure of a beta-LS. The, 1.95 A resolution structure of Streptomyces clavuligerus beta-LS provides, a fully resolved view of the active site in which substrate, closely, related ATP analog alpha,beta-methyleneadenosine 5'-triphosphate (AMP-CPP), and a single Mg2+ ion are present. A high degree of substrate, preorganization is observed. Comparison to Escherichia coli AS-B reveals, the evolutionary changes that have taken place in beta-LS that impede, interdomain reaction, which is essential in AS-B, and that accommodate, beta-lactam formation. The structural data provide the opportunity to, alter the synthetic potential of beta-LS, perhaps leading to the creation, of new beta-lactamase inhibitors and beta-lactam antibiotics.
<StructureSection load='1jgt' size='340' side='right'caption='[[1jgt]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1jgt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JGT FirstGlance]. <br>
1JGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with MG, APC, CMA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JGT OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=CMA:N2-(CARBOXYETHYL)-L-ARGININE'>CMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jgt OCA], [https://pdbe.org/1jgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jgt RCSB], [https://www.ebi.ac.uk/pdbsum/1jgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jgt ProSAT]</span></td></tr>
Structure of beta-lactam synthetase reveals how to synthesize antibiotics instead of asparagine., Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC, Nat Struct Biol. 2001 Aug;8(8):684-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11473258 11473258]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/BLS_STRCL BLS_STRCL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jg/1jgt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jgt ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
[[Category: Bachmann, B.O.]]
[[Category: Bachmann BO]]
[[Category: Miller, M.T.]]
[[Category: Miller MT]]
[[Category: Rosenzweig, A.C.]]
[[Category: Rosenzweig AC]]
[[Category: Townsend, C.A.]]
[[Category: Townsend CA]]
[[Category: APC]]
[[Category: CMA]]
[[Category: GOL]]
[[Category: MG]]
[[Category: ampcpp]]
[[Category: asparagine synthetase]]
[[Category: beta-lactam synthetase]]
[[Category: carboxyethylarginine]]
[[Category: cea]]
[[Category: clavulanic acid]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:14:30 2007''

Latest revision as of 07:39, 7 February 2024

CRYSTAL STRUCTURE OF BETA-LACTAM SYNTHETASE

1jgt, resolution 1.95Å

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