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New page: left|200px<br /><applet load="1ji6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ji6, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1ji6.jpg|left|200px]]<br /><applet load="1ji6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ji6, resolution 2.4&Aring;" />
'''CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS==
The coleopteran-active delta-endotoxin Cry3Bb1 from Bacillus thuringiensis, (Bt) strain EG7231 is uniquely toxic to Diabrotica undecimpunctata, the, Southern corn rootworm, while retaining activity against Leptinotarsa, decemlineata, the Colorado potato beetle. The crystal structure of the, delta-endotoxin Cry3Bb1 has been refined using data collected to 2.4 A, resolution, with a residual R factor of 17.5% and an R(free) of 25.3%. The, structure is made up of three domains: I, a seven-helix bundle (residues, 64-294); II, a three-sheet domain (residues 295-502); and III, a, beta-sandwich domain (residues 503-652). The monomers in the orthorhombic, C222(1) crystal lattice form a dimeric quaternary structure across a, crystallographic twofold axis, with a channel formed involving, interactions between domains I and III. There are 23 hydrogen bonds, between the two monomers conferring structural stability on the dimer. It, has been demonstrated that Cry3Bb1 and the similar toxin Cry3A form, oligomers in solution. The structural results presented here indicate that, the interactions between domains I and III could be responsible for the, initial higher order structure and have implications for the biological, activity of these toxins. There are seven additional single amino-acid, residues in the sequence of Cry3Bb1 compared with that of Cry3A; one in, domain I, two in domain II and four in domain III, which also shows the, largest conformational difference between the two proteins. These changes, can be implicated in the selectivity differences noted for these two, delta-endotoxins.
<StructureSection load='1ji6' size='340' side='right'caption='[[1ji6]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [https://pdbe.org/1ji6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ji6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CR3BB_BACTU CR3BB_BACTU] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera. Has moderate level of toxicity to southern corn rootworm.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1ji6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ji6 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JI6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA].
*[[Delta-endotoxin|Delta-endotoxin]]
 
*[[Pesticidal crystal protein|Pesticidal crystal protein]]
==Reference==
__TOC__
Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis., Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11468393 11468393]
</StructureSection>
[[Category: Bacillus thuringiensis]]
[[Category: Bacillus thuringiensis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cody, V.]]
[[Category: Cody V]]
[[Category: cry3bb1]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:17:10 2007''

Latest revision as of 07:39, 7 February 2024

CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS

1ji6, resolution 2.40Å

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