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New page: left|200px<br /><applet load="1jjw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jjw, resolution 1.90Å" /> '''Structure of Haemoph...
 
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[[Image:1jjw.gif|left|200px]]<br /><applet load="1jjw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1jjw, resolution 1.90&Aring;" />
'''Structure of Haemophilus influenzae HslV Protein at 1.9 A Resolution'''<br />


==Overview==
==Structure of Haemophilus influenzae HslV Protein at 1.9 A Resolution==
The structure of the Haemophilus influenzae HslV protease of the HslUV, 'prokaryotic proteasome' has been solved by molecular replacement and, refined with data to 1.9 A resolution. The protease is a 'double donut' of, hexameric rings; two alternative sets of intermolecular interactions, between protomers in the rings result in 'quasi-equivalent' packing within, the assembly. Anomalous scattering data from crystals with potassium, present in the mother liquor reveal a K(+) ion bound with octahedral, coordination near the active-site Thr1 residue. The site also binds Na(+), ions and is likely to bind Mg(2+), suggesting that monovalent and divalent, metal ions may influence the catalytic activity of the protease.
<StructureSection load='1jjw' size='340' side='right'caption='[[1jjw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jjw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjw OCA], [https://pdbe.org/1jjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjw RCSB], [https://www.ebi.ac.uk/pdbsum/1jjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HSLV_HAEIN HSLV_HAEIN] Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.[HAMAP-Rule:MF_00248]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jjw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jjw ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the Haemophilus influenzae HslV protease of the HslUV 'prokaryotic proteasome' has been solved by molecular replacement and refined with data to 1.9 A resolution. The protease is a 'double donut' of hexameric rings; two alternative sets of intermolecular interactions between protomers in the rings result in 'quasi-equivalent' packing within the assembly. Anomalous scattering data from crystals with potassium present in the mother liquor reveal a K(+) ion bound with octahedral coordination near the active-site Thr1 residue. The site also binds Na(+) ions and is likely to bind Mg(2+), suggesting that monovalent and divalent metal ions may influence the catalytic activity of the protease.


==About this Structure==
Structure of Haemophilus influenzae HslV protein at 1.9 A resolution, revealing a cation-binding site near the catalytic site.,Sousa MC, McKay DB Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1950-4. Epub 2001, Nov 21. PMID:11717526<ref>PMID:11717526</ref>
1JJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JJW OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of Haemophilus influenzae HslV protein at 1.9 A resolution, revealing a cation-binding site near the catalytic site., Sousa MC, McKay DB, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1950-4. Epub 2001, Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11717526 11717526]
</div>
<div class="pdbe-citations 1jjw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: McKay, D.B.]]
[[Category: McKay DB]]
[[Category: Sousa, M.C.]]
[[Category: Sousa MC]]
[[Category: K]]
[[Category: k anomalous scattering]]
[[Category: quasi-equivalent packing]]
 
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