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New page: left|200px<br /><applet load="1jqm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jqm" /> '''Fitting of L11 protein and elongation factor...
 
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[[Image:1jqm.gif|left|200px]]<br /><applet load="1jqm" size="450" color="white" frame="true" align="right" spinBox="true"
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'''Fitting of L11 protein and elongation factor G (EF-G) in the cryo-em map of e. coli 70S ribosome bound with EF-G, GDP and fusidic acid'''<br />


==Overview==
==Fitting of L11 protein and elongation factor G (EF-G) in the cryo-em map of e. coli 70S ribosome bound with EF-G, GDP and fusidic acid==
L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit, of the Escherichia coli ribosome. Because of the flexible nature of the, region, recent X-ray crystallographic studies of the 50 S subunit failed, to locate the N-terminal domain of the protein. We have determined the, position of the complete L11 protein by comparing a three-dimensional, cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant, lacking ribosomal protein L11, with the three-dimensional map of the, wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA, complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G, intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal, domain of L11 (where the antibiotic thiostrepton binds), causing the, N-terminal domain to move and thereby inducing the formation of the, arc-like connection with the G' domain of EF-G. The results provide a new, insight into the mechanism of EF-G-dependent translocation.
<SX load='1jqm' size='340' side='right' viewer='molstar' caption='[[1jqm]], [[Resolution|resolution]] 18.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jqm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 18&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqm OCA], [https://pdbe.org/1jqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqm RCSB], [https://www.ebi.ac.uk/pdbsum/1jqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA] This protein binds directly to 23S ribosomal RNA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jqm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jqm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JQM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JQM OCA].
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
 
*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
==Reference==
__TOC__
Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11518530 11518530]
</SX>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Agrawal, R.K.]]
[[Category: Agrawal RK]]
[[Category: Frank, J.]]
[[Category: Frank J]]
[[Category: Linde, J.]]
[[Category: Linde J]]
[[Category: Nierhaus, K.H.]]
[[Category: Nierhaus KH]]
[[Category: Segupta, J.]]
[[Category: Segupta J]]
[[Category: 70s e.coli ribosome]]
[[Category: cryo-em]]
[[Category: ef-g]]
[[Category: gdp state]]
[[Category: l11]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:29:16 2007''
Retrieved from "https://proteopedia.org/1jqm"