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New page: left|200px<br /><applet load="1jy8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jy8, resolution 2.5Å" /> '''2C-methyl-D-erythrito...
 
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[[Image:1jy8.jpg|left|200px]]<br /><applet load="1jy8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1jy8, resolution 2.5&Aring;" />
'''2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF)'''<br />


==Overview==
==2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF)==
Isoprenoids are biosynthesized from isopentenyl diphosphate and the, isomeric dimethylallyl diphosphate via the mevalonate pathway or a, mevalonate-independent pathway that was identified during the last decade., The non-mevalonate pathway is present in many bacteria, some algae and in, certain protozoa such as the malaria parasite Plasmodium falciparum and in, the plastids of higher plants, but not in mammals and archaea. Therefore, these enzymes have been recognised as promising drug targets. We report, the crystal structure of Escherichia coli 2C-, methyl-d-erythritol-2,4-cyclodiphosphate synthase (IspF), which converts, 4-diphosphocytidyl-2C-methyl-d-erythritol 2-phosphate into, 2C-methyl-d-erythritol 2,4-cyclodiphosphate and CMP in a Mg-dependent, reaction. The protein forms homotrimers that tightly bind one zinc ion per, subunit at the active site, which helps to position the substrate for, direct attack of the 2-phosphate group on the beta-phosphate.
<StructureSection load='1jy8' size='340' side='right'caption='[[1jy8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jy8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JY8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CDI:2C-METHYL-D-ERYTHRITOL+2,4-CYCLODIPHOSPHATE'>CDI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jy8 OCA], [https://pdbe.org/1jy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jy8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jy8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ISPF_ECOLI ISPF_ECOLI] Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). Also converts 4-diphosphocytidyl-2-C-methyl-D-erythritol into 2-C-methyl-D-erythritol 3,4-cyclophosphate and CMP.<ref>PMID:10694574</ref> <ref>PMID:22839733</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jy/1jy8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jy8 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, C5P and CDI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JY8 OCA].
*[[MECDP synthase 3D structures|MECDP synthase 3D structures]]
 
== References ==
==Reference==
<references/>
Structure of 2C-methyl-d-erythritol-2,4-cyclodiphosphate synthase involved in mevalonate-independent biosynthesis of isoprenoids., Steinbacher S, Kaiser J, Wungsintaweekul J, Hecht S, Eisenreich W, Gerhardt S, Bacher A, Rohdich F, J Mol Biol. 2002 Feb 8;316(1):79-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11829504 11829504]
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bacher, A.]]
[[Category: Bacher A]]
[[Category: Eisenreich, W.]]
[[Category: Eisenreich W]]
[[Category: Gerhardt, S.]]
[[Category: Gerhardt S]]
[[Category: Hecht, S.]]
[[Category: Hecht S]]
[[Category: Kaiser, J.]]
[[Category: Kaiser J]]
[[Category: Rohdich, F.]]
[[Category: Rohdich F]]
[[Category: Steinbacher, S.]]
[[Category: Steinbacher S]]
[[Category: Wungsintaweekul, J.]]
[[Category: Wungsintaweekul J]]
[[Category: C5P]]
[[Category: CDI]]
[[Category: ZN]]
[[Category: isoprenoid biosynthesis; terpenes; malaria; drug design]]
 
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Retrieved from "https://proteopedia.org/1jy8"