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New page: left|200px<br /><applet load="1k0r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k0r, resolution 1.70Å" /> '''Crystal Structure of...
 
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[[Image:1k0r.gif|left|200px]]<br /><applet load="1k0r" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k0r, resolution 1.70&Aring;" />
'''Crystal Structure of Mycobacterium tuberculosis NusA'''<br />


==Overview==
==Crystal Structure of Mycobacterium tuberculosis NusA==
Mycobacterium tuberculosis is the cause of tuberculosis in humans, a, disease that affects over a one-third of the world's population. This, slow-growing pathogen has only one ribosomal RNA operon, thus making its, transcriptional apparatus a fundamentally interesting target for drug, discovery. NusA binds to RNA polymerase and modulates several of the, ribosomal RNA transcriptional processes. Here, we report the crystal, structure of NusA, and reveal that the molecule consists of four domains., They are organised as two distinct entities. The N-terminal domain, (residues 1 to 99) that resembles the B chain of the Rad50cd ATP binding, cassette-ATPase (ABC-ATPase) and a C-terminal module (residues 108 to 329), consisting of a ribosomal S1 protein domain followed by two K homology, domains. The S1 and KH domains are tightly integrated together to form an, extensive RNA-binding structure, but are flexibly tethered to the, N-terminal domain. The molecule's surfaces and architecture provide, insights into RNA and polymerase interactions and the mechanism of pause, site discrimination. They also allow us to rationalize certain, termination-defective and cold shock-sensitive mutations in the nusA gene, that have been studied in Escherichia coli.
<StructureSection load='1k0r' size='340' side='right'caption='[[1k0r]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1k0r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0R FirstGlance]. <br>
1K0R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K0R OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0r OCA], [https://pdbe.org/1k0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0r RCSB], [https://www.ebi.ac.uk/pdbsum/1k0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0r ProSAT]</span></td></tr>
Crystal structure of the transcription elongation/anti-termination factor NusA from Mycobacterium tuberculosis at 1.7 A resolution., Gopal B, Haire LF, Gamblin SJ, Dodson EJ, Lane AN, Papavinasasundaram KG, Colston MJ, Dodson G, J Mol Biol. 2001 Dec 14;314(5):1087-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11743725 11743725]
</table>
== Function ==
[https://www.uniprot.org/uniprot/NUSA_MYCTU NUSA_MYCTU] Participates in both transcription termination and antitermination (By similarity).[HAMAP-Rule:MF_00945]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0r_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0r ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Colston MJ]]
[[Category: Colston, M.J.]]
[[Category: Dodson EJ]]
[[Category: Dodson, E.J.]]
[[Category: Dodson G]]
[[Category: Dodson, G.]]
[[Category: Gamblin SJ]]
[[Category: Gamblin, S.J.]]
[[Category: Gopal B]]
[[Category: Gopal, B.]]
[[Category: Haire LF]]
[[Category: Haire, L.F.]]
[[Category: Lane AN]]
[[Category: Lane, A.N.]]
[[Category: Papavinasasundaram KG]]
[[Category: Papavinasasundaram, K.G.]]
[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
[[Category: SO4]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: s1 domain]]
[[Category: structural genomics]]
[[Category: tb structural genomics consortium]]
[[Category: tbsgc]]
[[Category: two component arrangement]]
[[Category: two k-homology domains.]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:45:34 2007''

Latest revision as of 07:43, 7 February 2024

Crystal Structure of Mycobacterium tuberculosis NusA

1k0r, resolution 1.70Å

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