1k1z: Difference between revisions
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New page: left|200px<br /><applet load="1k1z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k1z" /> '''Solution structure of N-terminal SH3 domain ... |
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== | ==Solution structure of N-terminal SH3 domain mutant(P33G) of murine Vav== | ||
The three-dimensional structure of the N-terminal SH3 domain (residues | <StructureSection load='1k1z' size='340' side='right'caption='[[1k1z]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1k1z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K1Z FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k1z OCA], [https://pdbe.org/1k1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k1z RCSB], [https://www.ebi.ac.uk/pdbsum/1k1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k1z ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/VAV_MOUSE VAV_MOUSE] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k1/1k1z_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k1z ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The three-dimensional structure of the N-terminal SH3 domain (residues 583-660) of murine Vav, which contains a tetra-proline sequence (Pro 607-Pro 610), was determined by NMR. The solution structure of the SH3 domain shows a typical SH3 fold, but it exists in two conformations due to cis-trans isomerization at the Gly614-Pro615 bond. The NMR structure of the P615G mutant, where Pro615 is replaced by glycine, reveals that the tetra-proline region is inserted into the RT-loop and binds to its own SH3 structure. The C-terminal SH3 domain of Grb2 specifically binds to the trans form of the N-terminal SH3 domain of Vav. The surface of Vav N-terminal SH3 which binds to Grb2 C-terminal SH3 was elucidated by chemical shift mapping experiments using NMR. The surface does not involve the tetra-proline region but involves the region comprising the n-src loop, the N-terminal and the C-terminal regions. This surface is located opposite to the tetra-proline containing region, consistent with that of our previous mutagenesis studies. | |||
Solution structure of N-terminal SH3 domain of Vav and the recognition site for Grb2 C-terminal SH3 domain.,Ogura K, Nagata K, Horiuchi M, Ebisui E, Hasuda T, Yuzawa S, Nishida M, Hatanaka H, Inagaki F J Biomol NMR. 2002 Jan;22(1):37-46. PMID:11885979<ref>PMID:11885979</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1k1z" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Ebisui E]] | |||
[[Category: Ebisui | [[Category: Hasuda T]] | ||
[[Category: Hasuda | [[Category: Hatanaka H]] | ||
[[Category: Hatanaka | [[Category: Horiuchi M]] | ||
[[Category: Horiuchi | [[Category: Inagaki F]] | ||
[[Category: Inagaki | [[Category: Nagata K]] | ||
[[Category: Nagata | [[Category: Nishida M]] | ||
[[Category: Nishida | [[Category: Ogura K]] | ||
[[Category: Ogura | [[Category: Yuzawa S]] | ||
[[Category: Yuzawa | |||
Latest revision as of 18:12, 29 May 2024
Solution structure of N-terminal SH3 domain mutant(P33G) of murine Vav
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