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New page: left|200px<br /><applet load="1k6a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k6a, resolution 1.14Å" /> '''Structural studies o...
 
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[[Image:1k6a.gif|left|200px]]<br /><applet load="1k6a" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k6a, resolution 1.14&Aring;" />
'''Structural studies on the mobility in the active site of the Thermoascus aurantiacus xylanase I'''<br />


==Overview==
==Structural studies on the mobility in the active site of the Thermoascus aurantiacus xylanase I==
The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX), has been investigated both biochemically and structurally. High resolution, crystallographic analyses at 291 K and 100 K of TAX complexes with, xylobiose show that the ligand is in its alpha anomeric conformation and, provide a rationale for specificity on p-nitrophenyl glycosides at the -1, and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in, family 10 are identified, which differ by the presence or absence of a, short helical stretch in the eighth betaalpha-loop of the TIM barrel, the, loop bearing Trp 275. This structural difference is discussed in the, context of Trp 275 mobility and xylanase function.
<StructureSection load='1k6a' size='340' side='right'caption='[[1k6a]], [[Resolution|resolution]] 1.14&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1k6a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1fxm 1fxm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K6A FirstGlance]. <br>
1K6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. This structure superseeds the now removed PDB entry 1FXM. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K6A OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.14&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6a OCA], [https://pdbe.org/1k6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k6a RCSB], [https://www.ebi.ac.uk/pdbsum/1k6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k6a ProSAT]</span></td></tr>
==Reference==
</table>
Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A., Lo Leggio L, Kalogiannis S, Eckert K, Teixeira SC, Bhat MK, Andrei C, Pickersgill RW, Larsen S, FEBS Lett. 2001 Dec 7;509(2):303-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11741607 11741607]
== Function ==
[[Category: Endo-1,4-beta-xylanase]]
[https://www.uniprot.org/uniprot/XYNA_THEAU XYNA_THEAU]
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k6a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k6a ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermoascus aurantiacus]]
[[Category: Thermoascus aurantiacus]]
[[Category: Andrei, C.]]
[[Category: Andrei C]]
[[Category: Bhat, M.K.]]
[[Category: Bhat MK]]
[[Category: Eckert, K.]]
[[Category: Eckert K]]
[[Category: Kalogiannis, S.]]
[[Category: Kalogiannis S]]
[[Category: Larsen, S.]]
[[Category: Larsen S]]
[[Category: Leggio, L.Lo.]]
[[Category: Lo Leggio L]]
[[Category: Pickersgill, R.W.]]
[[Category: Pickersgill RW]]
[[Category: Teixeira, S.C.M.]]
[[Category: Teixeira SCM]]
[[Category: active site mobility]]
[[Category: alternate conformations]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:53:12 2007''

Latest revision as of 07:58, 3 April 2024

Structural studies on the mobility in the active site of the Thermoascus aurantiacus xylanase I

1k6a, resolution 1.14Å

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