2fbs: Difference between revisions

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{{Seed}}
[[Image:2fbs.png|left|200px]]


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==Solution structure of the LL-37 core peptide bound to detergent micelles==
The line below this paragraph, containing "STRUCTURE_2fbs", creates the "Structure Box" on the page.
<StructureSection load='2fbs' size='340' side='right'caption='[[2fbs]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2fbs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBS FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
{{STRUCTURE_2fbs|  PDB=2fbs  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbs OCA], [https://pdbe.org/2fbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbs RCSB], [https://www.ebi.ac.uk/pdbsum/2fbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAMP_HUMAN CAMP_HUMAN] Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.<ref>PMID:16637646</ref> <ref>PMID:18818205</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To understand the structure and activity relationship of human LL-37, a series of peptide fragments was designed. The N-terminal fragment, LL-37(1-12), was not active, while the C-terminal fragment, LL-37(13-37), killed Escherichia coli, as well as drug-sensitive and drug-resistant cancer cells. A 13-residue core antibacterial and anticancer peptide, corresponding to residues 17-29 of LL-37, was identified based on total correlated spectroscopy by trimming nonessential regions (TOCSY-trim). Because LL-37 acts on bacterial membranes, three-dimensional structures of its fragments were determined in micelles by NMR, including structural refinement by natural abundance 15N and 13C chemical shifts. Aromatic-aromatic interactions in the N-terminal fragment were proposed to be essential for LL-37 aggregation. The LL-37 core peptide adopts a similar structure in the micelles of SDS or dioctanoyl phosphatidylglycerol. This structure is retained in the C-terminal fragment LL-37(13-37) and very likely in intact LL-37 based on peptide-aided signal assignments. The higher antibacterial activity of the LL-37 core peptide than aurein 1.2 was attributed to additional cationic residues. To achieve selective membrane targeting, D-amino acids were incorporated into LL-37(17-32). While the D-peptide showed similar antibacterial activity to the L-diastereomer, it lost toxicity to human cells. Structural analysis revealed hydrophobic defects in the new amphipathic structure of the D-peptide, leading to a much shorter retention time on a reversed-phase HPLC column. It is proposed that hydrophobic defects as a result of incoherent hydrophobic packing provide a structural basis for the improvement in cell selectivity of the LL-37 fragment.


===Solution structure of the LL-37 core peptide bound to detergent micelles===
Solution structures of human LL-37 fragments and NMR-based identification of a minimal membrane-targeting antimicrobial and anticancer region.,Li X, Li Y, Han H, Miller DW, Wang G J Am Chem Soc. 2006 May 3;128(17):5776-85. PMID:16637646<ref>PMID:16637646</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2fbs" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16637646 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16637646}}
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</StructureSection>
==About this Structure==
[[Category: Homo sapiens]]
2FBS is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBS OCA].
[[Category: Large Structures]]
 
[[Category: Li X]]
==Reference==
[[Category: Wang G]]
Solution structures of human LL-37 fragments and NMR-based identification of a minimal membrane-targeting antimicrobial and anticancer region., Li X, Li Y, Han H, Miller DW, Wang G, J Am Chem Soc. 2006 May 3;128(17):5776-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16637646 16637646]
[[Category: Single protein]]
[[Category: Li, X.]]
[[Category: Li, Y.]]
[[Category: Wang, G.]]
[[Category: Amphipathic helix]]
[[Category: Antimicrobial peptide]]
[[Category: Host defense peptide]]
[[Category: Ll-37]]
 
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