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New page: left|200px<br /><applet load="1kfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kfi, resolution 2.4Å" /> '''Crystal Structure of ...
 
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[[Image:1kfi.jpg|left|200px]]<br /><applet load="1kfi" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1kfi, resolution 2.4&Aring;" />
'''Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium'''<br />


==Overview==
==Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium==
During exocytosis of dense-core secretory vesicles (trichocysts) in, Paramecium, the protein pp63/parafusin (pp63/pf) is transiently, dephosphorylated. We report here the structures of two crystal forms of, one isoform of this protein which has a high degree of homology with, rabbit phosphoglucomutase, whose structure has been reported. As expected, both proteins possess highly similar structures, showing the same four, domains forming two lobes with an active-site crevice in between. The two, X-ray structures that we report here were determined after crystallization, in the presence of sulfate and tartrate, and show the lobes arranged as a, closed and an open conformation, respectively. While both conformations, possess a bound divalent cation, only the closed (sulfate-bound), conformation shows bound sulfate ions in the "phosphate-transfer site", near the catalytic serine residue and in the "phosphate-binding site"., Comparison with the open form shows that the latter dianion is placed in, the centre of three arginine residues, one contributed by subunit II and, two by subunit IV, suggesting that it causes a contraction of the arginine, triangle, which establishes the observed conformational closure of the, lobes. It is therefore likely that the closed conformation forms only when, a phosphoryl group is bound to the phosphate-binding site. The previously, published structure of rabbit phosphoglucomutase is intermediate between, these two conformers. Several of the known reversible phosphorylation, sites of pp63/pf-1 are at positions critical for transition between the, conformations and for binding of the ligands and thus give hints as to, possible roles of pp63/pf-1 in the course of exocytosis.
<StructureSection load='1kfi' size='340' side='right'caption='[[1kfi]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1kfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_tetraurelia Paramecium tetraurelia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KFI FirstGlance]. <br>
1KFI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_tetraurelia Paramecium tetraurelia] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoglucomutase Phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.2 5.4.2.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KFI OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kfi OCA], [https://pdbe.org/1kfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kfi RCSB], [https://www.ebi.ac.uk/pdbsum/1kfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kfi ProSAT]</span></td></tr>
Crystal structure analysis of the exocytosis-sensitive phosphoprotein, pp63/parafusin (phosphoglucomutase), from Paramecium reveals significant conformational variability., Muller S, Diederichs K, Breed J, Kissmehl R, Hauser K, Plattner H, Welte W, J Mol Biol. 2002 Jan 11;315(2):141-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11779235 11779235]
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGM1_PARTE PGM1_PARTE] May be involved in membrane fusion in exocytosis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/1kfi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kfi ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Paramecium tetraurelia]]
[[Category: Paramecium tetraurelia]]
[[Category: Phosphoglucomutase]]
[[Category: Breed J]]
[[Category: Single protein]]
[[Category: Diederichs K]]
[[Category: Breed, J.]]
[[Category: Hauser K]]
[[Category: Diederichs, K.]]
[[Category: Kissmehl R]]
[[Category: Hauser, K.]]
[[Category: Mueller S]]
[[Category: Kissmehl, R.]]
[[Category: Plattner H]]
[[Category: Mueller, S.]]
[[Category: Welte W]]
[[Category: Plattner, H.]]
[[Category: Welte, W.]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: exocytosis]]
[[Category: parafusin]]
[[Category: phosphoprotein pp63]]
 
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