2yz1: Difference between revisions

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{{Seed}}
[[Image:2yz1.png|left|200px]]


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==Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha==
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<StructureSection load='2yz1' size='340' side='right'caption='[[2yz1]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2yz1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YZ1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yz1 OCA], [https://pdbe.org/2yz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yz1 RCSB], [https://www.ebi.ac.uk/pdbsum/2yz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yz1 ProSAT]</span></td></tr>
{{STRUCTURE_2yz1|  PDB=2yz1  |  SCENE=  }}
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yz/2yz1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2yz1 ConSurf].
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
SRC homology 2 domain-containing protein tyrosine phosphatase substrate 1 (SHPS-1 or SIRP alpha/BIT) is an immunoglobulin (Ig) superfamily transmembrane receptor and a member of the signal regulatory protein (SIRP) family involved in cell-cell interaction. SHPS-1 binds to its ligand CD47 to relay an inhibitory signal for cellular responses, whereas SIRPbeta, an activating member of the same family, does not bind to CD47 despite sharing a highly homologous ligand-binding domain with SHPS-1. To address the molecular basis for specific CD47 recognition by SHPS-1, we present the crystal structure of the ligand-binding domain of murine SHPS-1 (mSHPS-1). Folding topology revealed that mSHPS-1 adopts an I2-set Ig fold, but its overall structure resembles IgV domains of antigen receptors, although it has an extended loop structure (C'E loop), which forms a dimer interface in the crystal. Site-directed mutagenesis studies of mSHPS-1 identified critical residues for CD47 binding including sites in the C'E loop and regions corresponding to complementarity-determining regions of antigen receptors. The structural and functional features of mSHPS-1 are consistent with the human SHPS-1 structure except that human SHPS-1 has an additional beta-strand D. These results suggest that the variable complementarity-determining region-like loop structures in the binding surface of SHPS-1 are generally required for ligand recognition in a manner similar to that of antigen receptors, which may explain the diverse ligand-binding specificities of SIRP family receptors.


===Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha===
Structural insight into the specific interaction between murine SHPS-1/SIRP alpha and its ligand CD47.,Nakaishi A, Hirose M, Yoshimura M, Oneyama C, Saito K, Kuki N, Matsuda M, Honma N, Ohnishi H, Matozaki T, Okada M, Nakagawa A J Mol Biol. 2008 Jan 18;375(3):650-60. Epub 2007 Nov 7. PMID:18045614<ref>PMID:18045614</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2yz1" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18045614}}, adds the Publication Abstract to the page
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18045614 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_18045614}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2YZ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YZ1 OCA].
 
==Reference==
Structural insight into the specific interaction between murine SHPS-1/SIRP alpha and its ligand CD47., Nakaishi A, Hirose M, Yoshimura M, Oneyama C, Saito K, Kuki N, Matsuda M, Honma N, Ohnishi H, Matozaki T, Okada M, Nakagawa A, J Mol Biol. 2008 Jan 18;375(3):650-60. Epub 2007 Nov 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18045614 18045614]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Nakaishi A]]
[[Category: Nakaishi, A.]]
[[Category: Alternative splicing]]
[[Category: Beta-sandwich]]
[[Category: Glycoprotein]]
[[Category: Immune system]]
[[Category: Immunoglobulin domain]]
[[Category: Membrane]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Phosphorylation]]
[[Category: Polymorphism]]
[[Category: Sh3-binding]]
[[Category: Structural genomic]]
[[Category: Transmembrane]]
 
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Retrieved from "https://proteopedia.org/2yz1"