4aig: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="4aig" size="450" color="white" frame="true" align="right" spinBox="true" caption="4aig, resolution 2.00Å" /> '''ADAMALYSIN II WITH P... |
No edit summary |
||
| (12 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | ==ADAMALYSIN II WITH PHOSPHONATE INHIBITOR== | ||
The search of reprolysin inhibitors offers the possibility of intervention | <StructureSection load='4aig' size='340' side='right'caption='[[4aig]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4aig]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crotalus_adamanteus Crotalus adamanteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AIG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FLX:N-[(FURAN-2-YL)CARBONYL]-(S)-LEUCYL-(R)-[1-AMINO-2(1H-INDOL-3-YL)ETHYL]-PHOSPHONIC+ACID'>FLX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aig OCA], [https://pdbe.org/4aig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aig RCSB], [https://www.ebi.ac.uk/pdbsum/4aig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aig ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/VM12_CROAD VM12_CROAD] Has no significant hemorrhagic activity, but inactivates serpins by limited proteolysis of their reactive-site loops. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/4aig_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4aig ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The search of reprolysin inhibitors offers the possibility of intervention against both matrixins and ADAMs. Here we report the crystal structure of the complex between adamalysin II, a member of the reprolysin family, and a phosphonate inhibitor modeled on an endogenous venom tripeptide. The inhibitor occupies the primed region of the cleavage site adopting a retro-binding mode. The phosphonate group ligates the zinc ion in an asymmetric bidentate mode and the adjacent Trp indole system partly fills the primary specificity subsite S1'. An adamalysin-based model of tumor necrosis factor-alpha-converting enzyme (TACE) reveals a smaller S1' pocket for this enzyme. | |||
2 angstrom X-ray structure of adamalysin II complexed with a peptide phosphonate inhibitor adopting a retro-binding mode.,Cirilli M, Gallina C, Gavuzzo E, Giordano C, Gomis-Ruth FX, Gorini B, Kress LF, Mazza F, Paradisi MP, Pochetti G, Politi V FEBS Lett. 1997 Dec 1;418(3):319-22. PMID:9428736<ref>PMID:9428736</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4aig" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Crotalus adamanteus]] | [[Category: Crotalus adamanteus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cirilli | [[Category: Cirilli M]] | ||
[[Category: Gavuzzo | [[Category: Gavuzzo E]] | ||
[[Category: Mazza | [[Category: Mazza F]] | ||
[[Category: Pochetti | [[Category: Pochetti G]] | ||
Latest revision as of 06:20, 17 October 2024
ADAMALYSIN II WITH PHOSPHONATE INHIBITOR
| ||||||||||||
