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New page: left|200px<br /><applet load="4rub" size="450" color="white" frame="true" align="right" spinBox="true" caption="4rub, resolution 2.7Å" /> '''A CRYSTAL FORM OF RIB...
 
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[[Image:4rub.gif|left|200px]]<br /><applet load="4rub" size="450" color="white" frame="true" align="right" spinBox="true"
caption="4rub, resolution 2.7&Aring;" />
'''A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE'''<br />


==Overview==
==A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE==
A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC, 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with, polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the, hanging-drop vapor diffusion technique from a protein solution containing, enzyme complexed with CO2, Mg2+, and the transition state analog, 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the, space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and, c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric, molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg, spacing and are suitable for X-ray structure determination.
<StructureSection load='4rub' size='340' side='right'caption='[[4rub]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rub]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RUB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rub OCA], [https://pdbe.org/4rub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rub RCSB], [https://www.ebi.ac.uk/pdbsum/4rub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rub ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RBS_TOBAC RBS_TOBAC] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ru/4rub_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4rub ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.


==About this Structure==
A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state.,Suh SW, Cascio D, Chapman MS, Eisenberg D J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:3681999<ref>PMID:3681999</ref>
4RUB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with MG, CAP and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4RUB OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state., Suh SW, Cascio D, Chapman MS, Eisenberg D, J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3681999 3681999]
</div>
<div class="pdbe-citations 4rub" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[RuBisCO 3D structures|RuBisCO 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
[[Category: Protein complex]]
[[Category: Cascio D]]
[[Category: Ribulose-bisphosphate carboxylase]]
[[Category: Curmi PMG]]
[[Category: Cascio, D.]]
[[Category: Eisenberg D]]
[[Category: Curmi, P.M.G.]]
[[Category: Schreuder H]]
[[Category: Eisenberg, D.]]
[[Category: Schreuder, H.]]
[[Category: CAP]]
[[Category: FMT]]
[[Category: MG]]
[[Category: lyase(carbon-carbon)]]
 
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