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New page: left|200px<br /><applet load="3mra" size="450" color="white" frame="true" align="right" spinBox="true" caption="3mra" /> '''M3 TRANSMEMBRANE SEGMENT OF ALPHA-SUBUNIT OF...
 
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[[Image:3mra.jpg|left|200px]]<br /><applet load="3mra" size="450" color="white" frame="true" align="right" spinBox="true"
caption="3mra" />
'''M3 TRANSMEMBRANE SEGMENT OF ALPHA-SUBUNIT OF NICOTINIC ACETYLCHOLINE RECEPTOR FROM TORPEDO CALIFORNICA, NMR, 15 STRUCTURES'''<br />


==Overview==
==M3 TRANSMEMBRANE SEGMENT OF ALPHA-SUBUNIT OF NICOTINIC ACETYLCHOLINE RECEPTOR FROM TORPEDO CALIFORNICA, NMR, 15 STRUCTURES==
The three-dimensional structure of a synthetic peptide corresponding to, the putative transmembrane segment M3 (amino acid residues 277-301) of the, alpha subunit of the nicotinic acetylcholine receptor from Torpedo, californica has been studied by means of two-dimensional 1H-NMR, spectroscopy in a chloroform/methanol (1:1) mixture containing 0.1 M, LiClO4. Complete resonance assignment has been performed using, double-quantum-filtered COSY (DQF-COSY), TOCSY and NOESY spectra. The, spatial structure has been calculated using the Diana program on the basis, of integrated intensities of NOESY spectra. HN-C(alpha)H and, HC(alpha)-C(beta)H spin-spin coupling constants. Residues 279-297 of M3, form a right-handed helix (root mean square deviation is 0.032 nm for, backbone atoms and 0.088 nm for all heavy atoms). The conformations of the, 17 side chains have been unambiguously determined. The obtained structure, is in accord with the photolabeling pattern of the membrane nicotinic, acetylcholine receptor (nAChR) which suggests alpha-helical structure of, M3 in the labeled portion [Blanton, M. P. &amp; Cohen, J. B. (1994), Biochemistry 33, 2859-2872].
<StructureSection load='3mra' size='340' side='right'caption='[[3mra]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mra]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetronarce_californica Tetronarce californica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MRA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MRA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mra OCA], [https://pdbe.org/3mra PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mra RCSB], [https://www.ebi.ac.uk/pdbsum/3mra PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mra ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACHA_TETCF ACHA_TETCF] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.


==About this Structure==
==See Also==
3MRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3MRA OCA].
*[[Acetyl choline receptor 3D structures|Acetyl choline receptor 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Spatial structure of the M3 transmembrane segment of the nicotinic acetylcholine receptor alpha subunit., Lugovskoy AA, Maslennikov IV, Utkin YN, Tsetlin VI, Cohen JB, Arseniev AS, Eur J Biochem. 1998 Jul 15;255(2):455-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9716388 9716388]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Tetronarce californica]]
[[Category: Torpedo californica]]
[[Category: Arseniev AS]]
[[Category: Arseniev, A.S.]]
[[Category: Cohen JB]]
[[Category: Cohen, J.B.]]
[[Category: Lugovskoy AA]]
[[Category: Lugovskoy, A.A.]]
[[Category: Maslennikov IV]]
[[Category: Maslennikov, I.V.]]
[[Category: Tsetlin VI]]
[[Category: Tsetlin, V.I.]]
[[Category: Utkin YN]]
[[Category: Utkin, Y.N.]]
[[Category: alpha-helix]]
[[Category: ion-channel]]
[[Category: membrane protein]]
[[Category: receptor]]
[[Category: transmembrane segment]]
[[Category: transmembrane segment m3 of nachr]]
 
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