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New page: left|200px<br /><applet load="1ku1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ku1, resolution 1.93Å" /> '''Crystal Structure of...
 
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[[Image:1ku1.jpg|left|200px]]<br /><applet load="1ku1" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ku1, resolution 1.93&Aring;" />
'''Crystal Structure of the Sec7 Domain of Yeast GEA2'''<br />


==Overview==
==Crystal Structure of the Sec7 Domain of Yeast GEA2==
Activation of small G proteins of the Arf family is initiated by guanine, nucleotide exchange factors whose catalytic Sec7 domain stimulates the, dissociation of the tightly bound GDP nucleotide. The exchange reaction, involves distinct sequential steps that can be trapped by the, noncompetitive inhibitor brefeldin A, by mutation of an invariant, catalytic glutamate, or by removal of guanine nucleotides. Arf-GDP retains, most characteristics of its GDP-bound form at the initial low-affinity, Arf-GDP-Sec7 step. It then undergoes large conformational changes toward, its GTP-bound form at the next step, and eventually dissociates GDP to, form a nucleotide-free high-affinity Arf-Sec7 complex at the last step., Thus, Arf proteins evolve through different conformations that must be, accommodated by Sec7 domains in the course of the reaction. Here the, contribution of the flexibility of Sec7 domains to the exchange reaction, was investigated with the crystal structure of the unbound Sec7 domain of, yeast Gea2. Comparison with Gea2 in complex with nucleotide-free Arf1, Delta 17 [Goldberg, J. (1998) Cell 95, 237-248] reveals that Arf induces, closure of the two subdomains that form the sides of its active site., Several residues that determine sensitivity to brefeldin A are involved in, interdomain and local movements, pointing to the importance of the, flexibility of Sec7 domains for the inhibition mechanism. Altogether, this, suggests a model for the initial steps of the exchange reaction where Arf, docks onto the C-terminal domain of the Sec7 domain before closure of the, N-terminal domain positions the catalytic glutamate to complete the, reaction.
<StructureSection load='1ku1' size='340' side='right'caption='[[1ku1]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ku1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KU1 FirstGlance]. <br>
1KU1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KU1 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ku1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ku1 OCA], [https://pdbe.org/1ku1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ku1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ku1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ku1 ProSAT]</span></td></tr>
==Reference==
</table>
Mechanism of domain closure of Sec7 domains and role in BFA sensitivity., Renault L, Christova P, Guibert B, Pasqualato S, Cherfils J, Biochemistry. 2002 Mar 19;41(11):3605-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11888276 11888276]
== Function ==
[https://www.uniprot.org/uniprot/GEA2_YEAST GEA2_YEAST] Activates the ARF proteins by exchanging bound GDP for free GTP.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1ku1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ku1 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Cherfils J]]
[[Category: Cherfils, J.]]
[[Category: Christova P]]
[[Category: Christova, P.]]
[[Category: Guibert B]]
[[Category: Guibert, B.]]
[[Category: Pasqualato S]]
[[Category: Pasqualato, S.]]
[[Category: Renault L]]
[[Category: Renault, L.]]
[[Category: arf small gtp-binding proteins]]
[[Category: guanine nucleotide exchange factor (gef)]]
[[Category: sec7 domain]]
 
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