1kuj: Difference between revisions
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New page: left|200px<br /><applet load="1kuj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kuj, resolution 2.00Å" /> '''Crystal structure of... |
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== | ==Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose== | ||
Evidence is presented that the specificity of jacalin, the seed lectin | <StructureSection load='1kuj' size='340' side='right'caption='[[1kuj]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1kuj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KUJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kuj OCA], [https://pdbe.org/1kuj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kuj RCSB], [https://www.ebi.ac.uk/pdbsum/1kuj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kuj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1kuj_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kuj ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin. | |||
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose.,Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090<ref>PMID:11988090</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1kuj" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Agglutinin 3D structures|Agglutinin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Artocarpus integer]] | [[Category: Artocarpus integer]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Astoul | [[Category: Astoul CH]] | ||
[[Category: Barre | [[Category: Barre A]] | ||
[[Category: Bourne | [[Category: Bourne Y]] | ||
[[Category: Menu-Bouaouiche L]] | |||
[[Category: Menu-Bouaouiche | [[Category: Peumans WJ]] | ||
[[Category: Peumans | [[Category: Rouge P]] | ||
[[Category: Rouge | [[Category: Van Damme EJM]] | ||
[[Category: | [[Category: Zamboni V]] | ||
[[Category: | |||
Latest revision as of 09:04, 16 August 2023
Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose
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