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New page: left|200px<br /><applet load="1kut" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kut, resolution 2.20Å" /> '''Structural Genomics,...
 
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[[Image:1kut.gif|left|200px]]<br /><applet load="1kut" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1kut, resolution 2.20&Aring;" />
'''Structural Genomics, Protein TM1243, (SAICAR synthetase)'''<br />


==Overview==
==Structural Genomics, Protein TM1243, (SAICAR synthetase)==
As a part of a structural genomics program, the 2.2 angstroms resolution, crystal structure of the PurC gene product from Thermotoga maritima has, been solved. This 26.2 kDa protein belongs to the, phophoribosylaminoimidazole-succinocarboxamide or SAICAR synthase family, of enzymes, the members of which are involved in de novo purine, biosynthesis. SAICAR synthase can be divided into three subdomains: two, alpha+beta regions exhibiting structural homology with ATP-binding, proteins and a carboxy-terminal subdomain of two alpha-helices. The, asymmetric unit contains two copies of the protein which are covalently, linked by a disulfide bond between Cys126(A) and Cys126(B). This, 230-amino-acid protein exhibits high structural homology with SAICAR, synthase from baker's yeast. The protein structure is described and, compared with that of the ATP-SAICAR synthase complex from yeast.
<StructureSection load='1kut' size='340' side='right'caption='[[1kut]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1kut]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KUT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kut OCA], [https://pdbe.org/1kut PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kut RCSB], [https://www.ebi.ac.uk/pdbsum/1kut PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kut ProSAT], [https://www.topsan.org/Proteins/MCSG/1kut TOPSAN]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1kut_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kut ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
As a part of a structural genomics program, the 2.2 angstroms resolution crystal structure of the PurC gene product from Thermotoga maritima has been solved. This 26.2 kDa protein belongs to the phophoribosylaminoimidazole-succinocarboxamide or SAICAR synthase family of enzymes, the members of which are involved in de novo purine biosynthesis. SAICAR synthase can be divided into three subdomains: two alpha+beta regions exhibiting structural homology with ATP-binding proteins and a carboxy-terminal subdomain of two alpha-helices. The asymmetric unit contains two copies of the protein which are covalently linked by a disulfide bond between Cys126(A) and Cys126(B). This 230-amino-acid protein exhibits high structural homology with SAICAR synthase from baker's yeast. The protein structure is described and compared with that of the ATP-SAICAR synthase complex from yeast.


==About this Structure==
Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer.,Zhang R, Skarina T, Evdokimova E, Edwards A, Savchenko A, Laskowski R, Cuff ME, Joachimiak A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):335-9. Epub 2006 Mar 25. PMID:16582479<ref>PMID:16582479</ref>
1KUT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Active as [http://en.wikipedia.org/wiki/Phosphoribosylaminoimidazolesuccinocarboxamide_synthase Phosphoribosylaminoimidazolesuccinocarboxamide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.6 6.3.2.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KUT OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer., Zhang R, Skarina T, Evdokimova E, Edwards A, Savchenko A, Laskowski R, Cuff ME, Joachimiak A, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):335-9. Epub 2006 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16582479 16582479]
</div>
[[Category: Phosphoribosylaminoimidazolesuccinocarboxamide synthase]]
<div class="pdbe-citations 1kut" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
 
==See Also==
*[[SAICAR synthetase|SAICAR synthetase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Beasley, S.]]
[[Category: Beasley S]]
[[Category: Edwards, A.]]
[[Category: Edwards A]]
[[Category: Joachimiak, A.]]
[[Category: Joachimiak A]]
[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]
[[Category: Savchenko A]]
[[Category: Savchenko, A.]]
[[Category: Skarina T]]
[[Category: Skarina, T.]]
[[Category: Zhang R]]
[[Category: Zhang, R.]]
[[Category: mcsg]]
[[Category: midwest center for structural genomics]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: saicar synthetase]]
[[Category: structural genomics]]
 
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