3por: Difference between revisions

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New page: left|200px<br /><applet load="3por" size="450" color="white" frame="true" align="right" spinBox="true" caption="3por, resolution 2.5Å" /> '''PORIN CONFORMATION IN...
 
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[[Image:3por.jpg|left|200px]]<br /><applet load="3por" size="450" color="white" frame="true" align="right" spinBox="true"
caption="3por, resolution 2.5&Aring;" />
'''PORIN CONFORMATION IN THE ABSENCE OF CALCIUM; REFINED STRUCTURE AT 2.5 ANGSTROMS RESOLUTION'''<br />


==Overview==
==PORIN CONFORMATION IN THE ABSENCE OF CALCIUM; REFINED STRUCTURE AT 2.5 ANGSTROMS RESOLUTION==
The crystal structure of porin from Rhodobacter capsulatus in the absence, of divalent calcium ions has been refined to convergence at a resolution, of 2.5 A using the simulated annealing refinement method. The final model, consists of all 301 amino acid residues, 77 solvent molecules, one, tris(hydroxymethyl)-aminomethane molecule and one unknown ligand modeled, as n-octyltetraoxyethylene. A superposition with the previously described, model containing three calcium ions showed structural changes at the, segment 108-116 of the inner loop beta 5-beta 6, and at loops beta 8-beta, 9 and beta 11-beta 12 at the extracellular side of the porin molecule., Evidence is presented that the conformational changes depend on the, presence or absence of calcium ions. A possible influence on porin, function is discussed.
<StructureSection load='3por' size='340' side='right'caption='[[3por]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3por]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3POR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3POR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3por FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3por OCA], [https://pdbe.org/3por PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3por RCSB], [https://www.ebi.ac.uk/pdbsum/3por PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3por ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PORI_RHOCA PORI_RHOCA] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/po/3por_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3por ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
3POR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus] with C8E and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3POR OCA].
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
 
*[[Porin 3D structures|Porin 3D structures]]
==Reference==
__TOC__
Porin conformation in the absence of calcium. Refined structure at 2.5 A resolution., Weiss MS, Schulz GE, J Mol Biol. 1993 Jun 5;231(3):817-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7685826 7685826]
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodobacter capsulatus]]
[[Category: Rhodobacter capsulatus]]
[[Category: Single protein]]
[[Category: Schulz GE]]
[[Category: Schulz, G.E.]]
[[Category: Weiss MS]]
[[Category: Weiss, M.S.]]
[[Category: C8E]]
[[Category: TRS]]
[[Category: integral membrane protein porin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:56:27 2007''

Latest revision as of 10:42, 21 February 2024

PORIN CONFORMATION IN THE ABSENCE OF CALCIUM; REFINED STRUCTURE AT 2.5 ANGSTROMS RESOLUTION

3por, resolution 2.50Å

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