1y29: Difference between revisions

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{{Seed}}
[[Image:1y29.png|left|200px]]


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==Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR==
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<StructureSection load='1y29' size='340' side='right'caption='[[1y29]]' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1y29]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyriopagopus_schmidti Cyriopagopus schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y29 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y29 OCA], [https://pdbe.org/1y29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y29 RCSB], [https://www.ebi.ac.uk/pdbsum/1y29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y29 ProSAT]</span></td></tr>
{{STRUCTURE_1y29|  PDB=1y29  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/TXH10_CYRSC TXH10_CYRSC] Reversibly blocks N-type calcium channels (Cav2.2/CACNA1B) in rat dorsal root ganglion cells (IC(50)=40 nM). Elicits no toxic symptoms in either vertebrates or invertebrates during a period of 48 hours post-injection, when it was assayed in vivo by direct injection into mice and cockroaches.<ref>PMID:16439354</ref>
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== Publication Abstract from PubMed ==
Huwentoxin-X (HWTX-X) is a novel peptide toxin, purified from the venom of the spider Ornithoctonus huwena. It comprises 28 amino acid residues including six cysteine residues as disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Its cDNA, determined by rapid amplification of 3' and 5' cDNA ends, encodes a 65-residue prepropeptide. HWTX-X shares low sequence homology with omega-conotoxins GVIA and MVIIA, two well known blockers of N-type Ca2+ channels. Nevertheless, whole cell studies indicate that it can block N-type Ca2+ channels in rat dorsal root ganglion cells (IC50 40 nm) and the blockage by HWTX-X is completely reversible. The rank order of specificity for N-type Ca2+ channels is GVIA approximately HWTX-X &gt; MVIIA. In contrast to GVIA and MVIIA, HWTX-X had no detectable effect on the twitch response of rat vas deferens to low frequency electrical stimulation, indicating that HWTX-X has different selectivity for isoforms of N-type Ca2+ channels, compared with GVIA or MVIIA. A comparison of the structures of HWTX-X and GVIA reveals that they not only adopt a common structural motif (inhibitor cystine knot), but also have a similar functional motif, a binding surface formed by the critical residue Tyr, and several basic residues. However, the dissimilarities of their binding surfaces provide some insights into their different selectivities for isoforms of N-type Ca2+ channels.


===Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR===
Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena.,Liu Z, Dai J, Dai L, Deng M, Hu Z, Hu W, Liang S J Biol Chem. 2006 Mar 31;281(13):8628-35. Epub 2006 Jan 26. PMID:16439354<ref>PMID:16439354</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
{{ABSTRACT_PUBMED_16439354}}
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</StructureSection>
==About this Structure==
[[Category: Cyriopagopus schmidti]]
1Y29 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA].
[[Category: Large Structures]]
 
[[Category: Liang S]]
==Reference==
[[Category: Liu Z]]
Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena., Liu Z, Dai J, Dai L, Deng M, Hu Z, Hu W, Liang S, J Biol Chem. 2006 Mar 31;281(13):8628-35. Epub 2006 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16439354 16439354]
[[Category: Single protein]]
[[Category: Liang, S.]]
[[Category: Liu, Z.]]
[[Category: A double-stranded beta-sheet]]
[[Category: Ick]]
[[Category: Knottin]]
 
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